PTPN1 monoclonal antibody, clone 4F8F11
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More Files
- More Functions
Western Blot (Cell lysate)
Western blot analysis of Lane 1: Jurkat cell and Lane 2: MCF-7 cell with PTPN1 monoclonal antibody.
Western Blot (Transfected lysate)
Western blot analysis of Lane 1: HEK293 cell; Lane 2: PTPN1-hIgGFc transfected HEK293 cell with PTPN1 monoclonal antibody.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Immunohistochemical staining of paraffin-embedded bladder cancer tissues with PTPN1 monoclonal antibody.
Immunocytochemistry
Immunocytochemical staining of Hela cells with PTPN1 monoclonal antibody (green). DRAQ5 fluorescent DNA dye (blue). Actin filaments labeled with Alexa Fluor-555 phalloidin (red).
Enzyme-linked Immunoabsorbent Assay
ELISA analysis of PTPN1 monoclonal antibody, clone 4F8F11.
Flow Cytometry
Flow cytometric analysis of Jurkat cells with PTPN1 monoclonal antibody (green) and negative control (red).
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Specification
Product Description
Mouse monoclonal antibody raised against recombinant human PTPN1.
Immunogen
Recombinant protein corresponding to amino acid 40-246 of human PTPN1 from E. coli.
Host
Mouse
Theoretical MW (kDa)
50
Reactivity
Human
Form
Liquid
Isotype
IgG1
Recommend Usage
ELISA (1:10000)
Western Blot (1:500-1:2000)
Immunohistochemistry (1:200-1:1000)
Immunocytochemistry (1:200-1:1000)
Flow Cytometry (1:200-1:400)
The optimal working dilution should be determined by the end user.Storage Buffer
In PBS (0.05% sodium azide).
Storage Instruction
Store at 4°C. For long term storage store at -20°C.
Aliquot to avoid repeated freezing and thawing.Note
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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Applications
Western Blot (Cell lysate)
Western blot analysis of Lane 1: Jurkat cell and Lane 2: MCF-7 cell with PTPN1 monoclonal antibody.Western Blot (Transfected lysate)
Western blot analysis of Lane 1: HEK293 cell; Lane 2: PTPN1-hIgGFc transfected HEK293 cell with PTPN1 monoclonal antibody.Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Immunohistochemical staining of paraffin-embedded bladder cancer tissues with PTPN1 monoclonal antibody.Immunocytochemistry
Immunocytochemical staining of Hela cells with PTPN1 monoclonal antibody (green). DRAQ5 fluorescent DNA dye (blue). Actin filaments labeled with Alexa Fluor-555 phalloidin (red).Enzyme-linked Immunoabsorbent Assay
ELISA analysis of PTPN1 monoclonal antibody, clone 4F8F11.Flow Cytometry
Flow cytometric analysis of Jurkat cells with PTPN1 monoclonal antibody (green) and negative control (red). -
Gene Info — PTPN1
Entrez GeneID
5770Gene Name
PTPN1
Gene Alias
PTP1B
Gene Description
protein tyrosine phosphatase, non-receptor type 1
Gene Ontology
HyperlinkGene Summary
The protein encoded by this gene is the founding member of the protein tyrosine phosphatase (PTP) family, which was isolated and identified based on its enzymatic activity and amino acid sequence. PTPs catalyze the hydrolysis of the phosphate monoesters specifically on tyrosine residues. Members of the PTP family share a highly conserved catalytic motif, which is essential for the catalytic activity. PTPs are known to be signaling molecules that regulate a variety of cellular processes including cell growth, differentiation, mitotic cycle, and oncogenic transformation. This PTP has been shown to act as a negative regulator of insulin signaling by dephosphorylating the phosphotryosine residues of insulin receptor kinase. This PTP was also reported to dephosphorylate epidermal growth factor receptor kinase, as well as JAK2 and TYK2 kinases, which implicated the role of this PTP in cell growth control, and cell response to interferon stimulation. [provided by RefSeq
Other Designations
OTTHUMP00000031266|non-receptor tyrosine phosphatase 1|protein tyrosine phosphatase 1B|protein tyrosine phosphatase, placental
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Interactome
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Disease
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