VIL1 monoclonal antibody, clone 3E5G11
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More Files
- More Functions
Western Blot (Cell lysate)
Western blot analysis using VIL1 monoclonal antibody against SW620 cell lysate.
Western Blot (Transfected lysate)
Western blot analysis of Lane 1: HEK293 cell; Lane 2: VIL1-hIgGFc transfected HEK293 cell with VIL1 monoclonal antibody.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Immunohistochemical staining of paraffin-embedded cervical cancer tissues with VIL1 monoclonal antibody.
Immunocytochemistry
Immunocytochemical staining of Hela cells with VIL1 monoclonal antibody (green). DRAQ5 fluorescent DNA dye (blue). Actin filaments labeled with Alexa Fluor-555 phalloidin (red).
Enzyme-linked Immunoabsorbent Assay
ELISA analysis of VIL1 monoclonal antibody, clone 3E5G11.
Flow Cytometry
Flow cytometric analysis of SW620 cells with VIL1 monoclonal antibody (green) and negative control (red).
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Specification
Product Description
Mouse monoclonal antibody raised against recombinant human VIL1.
Immunogen
Recombinant protein corresponding to amino acid 1-209 of human VIL1 from E. coli.
Host
Mouse
Theoretical MW (kDa)
92.7
Reactivity
Human
Form
Liquid
Isotype
IgG1
Recommend Usage
ELISA (1:10000)
Western Blot (1:500-1:2000)
Immunohistochemistry (1:200-1:1000)
Immunocytochemistry (1:200-1:1000)
Flow Cytometry (1:200-1:400)
The optimal working dilution should be determined by the end user.Storage Buffer
In PBS (0.05% sodium azide).
Storage Instruction
Store at 4°C. For long term storage store at -20°C.
Aliquot to avoid repeated freezing and thawing.Note
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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Applications
Western Blot (Cell lysate)
Western blot analysis using VIL1 monoclonal antibody against SW620 cell lysate.Western Blot (Transfected lysate)
Western blot analysis of Lane 1: HEK293 cell; Lane 2: VIL1-hIgGFc transfected HEK293 cell with VIL1 monoclonal antibody.Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Immunohistochemical staining of paraffin-embedded cervical cancer tissues with VIL1 monoclonal antibody.Immunocytochemistry
Immunocytochemical staining of Hela cells with VIL1 monoclonal antibody (green). DRAQ5 fluorescent DNA dye (blue). Actin filaments labeled with Alexa Fluor-555 phalloidin (red).Enzyme-linked Immunoabsorbent Assay
ELISA analysis of VIL1 monoclonal antibody, clone 3E5G11.Flow Cytometry
Flow cytometric analysis of SW620 cells with VIL1 monoclonal antibody (green) and negative control (red). -
Gene Info — VIL1
Entrez GeneID
7429Gene Name
VIL1
Gene Alias
D2S1471, VIL
Gene Description
villin 1
Omim ID
193040Gene Ontology
HyperlinkGene Summary
This gene encodes a member of a family of calcium-regulated actin-binding proteins. This protein represents a dominant part of the brush border cytoskeleton which functions in the capping, severing, and bundling of actin filaments. Two mRNAs of 2.7 kb and 3.5 kb have been observed; they result from utilization of alternate poly-adenylation signals present in the terminal exon. [provided by RefSeq
Other Designations
OTTHUMP00000164145
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Interactome
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