XRCC6 monoclonal antibody, clone 7A9E7
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More Files
- More Functions
Western Blot (Cell lysate)
Western blot analysis of Lane 1: PC-2 cell; Lane 2: A549 cell; Lane 3: A431 cell; Lane 4: HepG2 cell and Lane 5: K562 cell with XRCC6 monoclonal antibody.
Western Blot (Transfected lysate)
Western blot analysis of Lane 1: HEK293 cell; Lane 2: XRCC6-hIgGFc transfected HEK293 cell with XRCC6 monoclonal antibody.
Immunocytochemistry
Immunocytochemical staining of MCF-7 cells with XRCC6 monoclonal antibody (green). DRAQ5 fluorescent DNA dye (blue). Actin filaments labeled with Alexa Fluor-555 phalloidin (red).
Enzyme-linked Immunoabsorbent Assay
ELISA analysis of XRCC6 monoclonal antibody, clone 7A9E7.
Flow Cytometry
Flow cytometric analysis of A431 cells with XRCC6 monoclonal antibody (green) and negative control (red).
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Specification
Product Description
Mouse monoclonal antibody raised against recombinant human XRCC6.
Immunogen
Recombinant protein corresponding to amino acid 6-214 of human XRCC6 from E. coli.
Host
Mouse
Theoretical MW (kDa)
69.8
Reactivity
Human
Form
Liquid
Isotype
IgG1
Recommend Usage
ELISA (1:10000)
Western Blot (1:500-1:2000)
Immunocytochemistry (1:200-1:1000)
Flow Cytometry (1:200-1:400)
The optimal working dilution should be determined by the end user.Storage Buffer
In PBS (0.05% sodium azide).
Storage Instruction
Store at 4°C. For long term storage store at -20°C.
Aliquot to avoid repeated freezing and thawing.Note
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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Applications
Western Blot (Cell lysate)
Western blot analysis of Lane 1: PC-2 cell; Lane 2: A549 cell; Lane 3: A431 cell; Lane 4: HepG2 cell and Lane 5: K562 cell with XRCC6 monoclonal antibody.Western Blot (Transfected lysate)
Western blot analysis of Lane 1: HEK293 cell; Lane 2: XRCC6-hIgGFc transfected HEK293 cell with XRCC6 monoclonal antibody.Immunocytochemistry
Immunocytochemical staining of MCF-7 cells with XRCC6 monoclonal antibody (green). DRAQ5 fluorescent DNA dye (blue). Actin filaments labeled with Alexa Fluor-555 phalloidin (red).Enzyme-linked Immunoabsorbent Assay
ELISA analysis of XRCC6 monoclonal antibody, clone 7A9E7.Flow Cytometry
Flow cytometric analysis of A431 cells with XRCC6 monoclonal antibody (green) and negative control (red). -
Gene Info — XRCC6
Entrez GeneID
2547Gene Name
XRCC6
Gene Alias
CTC75, CTCBF, G22P1, KU70, ML8, TLAA
Gene Description
X-ray repair complementing defective repair in Chinese hamster cells 6
Omim ID
152690Gene Ontology
HyperlinkGene Summary
The p70/p80 autoantigen is a nuclear complex consisting of two subunits with molecular masses of approximately 70 and 80 kDa. The complex functions as a single-stranded DNA-dependent ATP-dependent helicase. The complex may be involved in the repair of nonhomologous DNA ends such as that required for double-strand break repair, transposition, and V(D)J recombination. High levels of autoantibodies to p70 and p80 have been found in some patients with systemic lupus erythematosus. [provided by RefSeq
Other Designations
ATP-dependent DNA helicase II, 70 kDa subunit|CTC box binding factor 75 kDa subunit|Ku autoantigen p70 subunit|Ku autoantigen, 70kDa|OTTHUMP00000028581|thyroid autoantigen 70kD (Ku antigen)|thyroid autoantigen 70kDa (Ku antigen)|thyroid-lupus autoantigen
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Interactome
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Pathway
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Disease
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