RPS6KA2 monoclonal antibody, clone 3C4C8
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More Files
- More Functions
Western Blot (Cell lysate)
Western blot analysis of Lane 1: Hela cell; Lane 2: A431 cell; Lane 3: HEK293 cell; Lane 4; Jurkat cell; Lane 5: HepG2 cell; Lane 6: MCF-7 cell and Lane 7: NIH/3T3 cell with RPS6KA2 monoclonal antibody.
Western Blot (Transfected lysate)
Western blot analysis of Lane 1: HEK293 cell; Lane 2: RPS6KA2-hIgGFc transfected HEK293 cell with RPS6KA2 monoclonal antibody.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Immunohistochemical staining of paraffin-embedded lung cancer tissues with RPS6KA2 monoclonal antibody.
Immunocytochemistry
Immunocytochemical staining of A431 cells with RPS6KA2 monoclonal antibody (green). DRAQ5 fluorescent DNA dye (blue). Actin filaments labeled with Alexa Fluor-555 phalloidin (red).
Enzyme-linked Immunoabsorbent Assay
ELISA analysis of RPS6KA2 monoclonal antibody, clone 3C4C8.
Flow Cytometry
Flow cytometric analysis of Hela cells with RPS6KA2 monoclonal antibody (green) and negative control (red).
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Specification
Product Description
Mouse monoclonal antibody raised against recombinant human RPS6KA2.
Immunogen
Recombinant protein corresponding to amino acid 415-734 of human RPS6KA2 from E. coli.
Host
Mouse
Theoretical MW (kDa)
83.2
Reactivity
Human, Mouse
Form
Liquid
Isotype
IgG1
Recommend Usage
ELISA (1:10000)
Western Blot (1:500-1:2000)
Immunohistochemistry (1:200-1:1000)
Immunocytochemistry (1:200-1:1000)
Flow Cytometry (1:200-1:400)
The optimal working dilution should be determined by the end user.Storage Buffer
In PBS (0.05% sodium azide).
Storage Instruction
Store at 4°C. For long term storage store at -20°C.
Aliquot to avoid repeated freezing and thawing.Note
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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Applications
Western Blot (Cell lysate)
Western blot analysis of Lane 1: Hela cell; Lane 2: A431 cell; Lane 3: HEK293 cell; Lane 4; Jurkat cell; Lane 5: HepG2 cell; Lane 6: MCF-7 cell and Lane 7: NIH/3T3 cell with RPS6KA2 monoclonal antibody.Western Blot (Transfected lysate)
Western blot analysis of Lane 1: HEK293 cell; Lane 2: RPS6KA2-hIgGFc transfected HEK293 cell with RPS6KA2 monoclonal antibody.Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Immunohistochemical staining of paraffin-embedded lung cancer tissues with RPS6KA2 monoclonal antibody.Immunocytochemistry
Immunocytochemical staining of A431 cells with RPS6KA2 monoclonal antibody (green). DRAQ5 fluorescent DNA dye (blue). Actin filaments labeled with Alexa Fluor-555 phalloidin (red).Enzyme-linked Immunoabsorbent Assay
ELISA analysis of RPS6KA2 monoclonal antibody, clone 3C4C8.Flow Cytometry
Flow cytometric analysis of Hela cells with RPS6KA2 monoclonal antibody (green) and negative control (red). -
Gene Info — RPS6KA2
Entrez GeneID
6196Gene Name
RPS6KA2
Gene Alias
HU-2, MAPKAPK1C, RSK, RSK3, S6K-alpha, S6K-alpha2, p90-RSK3, pp90RSK3
Gene Description
ribosomal protein S6 kinase, 90kDa, polypeptide 2
Omim ID
601685Gene Ontology
HyperlinkGene Summary
This gene encodes a member of the RSK (ribosomal S6 kinase) family of serine/threonine kinases. This kinase contains 2 non-identical kinase catalytic domains and phosphorylates various substrates, including members of the mitogen-activated kinase (MAPK) signalling pathway. The activity of this protein has been implicated in controlling cell growth and differentiation. Alternate transcriptional splice variants, encoding different isoforms, have been characterized. [provided by RefSeq
Other Designations
ribosomal S6 kinase 3|ribosomal protein S6 kinase alpha 2|ribosomal protein S6 kinase, 90kD, polypeptide 2
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Interactome
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Pathway
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Disease
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