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Last updated: 2022/1/16
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RPS6KA2 monoclonal antibody, clone 3C4C8 

  • Catalog # : MAB16615
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  • Specification
  • Product Description:
  • Mouse monoclonal antibody raised against recombinant human RPS6KA2.
  • Immunogen:
  • Recombinant protein corresponding to amino acid 415-734 of human RPS6KA2 from E. coli.
  • Host:
  • Mouse
  • Theoretical MW (kDa):
  • 83.2
  • Reactivity:
  • Human, Mouse
  • Form:
  • Liquid
  • Isotype:
  • IgG1
  • Recommend Usage:
  • ELISA (1:10000)
    Western Blot (1:500-1:2000)
    Immunohistochemistry (1:200-1:1000)
    Immunocytochemistry (1:200-1:1000)
    Flow Cytometry (1:200-1:400)
    The optimal working dilution should be determined by the end user.
  • Storage Buffer:
  • In PBS (0.05% sodium azide).
  • Storage Instruction:
  • Store at 4°C. For long term storage store at -20°C.
    Aliquot to avoid repeated freezing and thawing.
  • Note:
  • This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
  • Applications
  • Western Blot (Cell lysate)
  • Western Blot (Cell lysate)
  • Western blot analysis of Lane 1: Hela cell; Lane 2: A431 cell; Lane 3: HEK293 cell; Lane 4; Jurkat cell; Lane 5: HepG2 cell; Lane 6: MCF-7 cell and Lane 7: NIH/3T3 cell with RPS6KA2 monoclonal antibody.
  • Western Blot (Transfected lysate)
  • Western Blot (Transfected lysate)
  • Western blot analysis of Lane 1: HEK293 cell; Lane 2: RPS6KA2-hIgGFc transfected HEK293 cell with RPS6KA2 monoclonal antibody.
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
  • Immunohistochemical staining of paraffin-embedded lung cancer tissues with RPS6KA2 monoclonal antibody.
  • Immunocytochemistry
  • Immunocytochemistry
  • Immunocytochemical staining of A431 cells with RPS6KA2 monoclonal antibody (green). DRAQ5 fluorescent DNA dye (blue). Actin filaments labeled with Alexa Fluor-555 phalloidin (red).
  • Enzyme-linked Immunoabsorbent Assay
  • Enzyme-linked Immunoabsorbent Assay
  • ELISA analysis of RPS6KA2 monoclonal antibody, clone 3C4C8.
  • Flow Cytometry
  • Flow Cytometry
  • Flow cytometric analysis of Hela cells with RPS6KA2 monoclonal antibody (green) and negative control (red).
  • Application Image
  • Western Blot (Cell lysate)
  • Western Blot (Cell lysate)
  • enlarge
  • Western Blot (Transfected lysate)
  • Western Blot (Transfected lysate)
  • enlarge
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
  • enlarge
  • Enzyme-linked Immunoabsorbent Assay
  • Enzyme-linked Immunoabsorbent Assay
  • enlarge
  • Gene Information
  • Entrez GeneID:
  • 6196
  • Gene Name:
  • RPS6KA2
  • Gene Alias:
  • HU-2,MAPKAPK1C,RSK,RSK3,S6K-alpha,S6K-alpha2,p90-RSK3,pp90RSK3
  • Gene Description:
  • ribosomal protein S6 kinase, 90kDa, polypeptide 2
  • Gene Summary:
  • This gene encodes a member of the RSK (ribosomal S6 kinase) family of serine/threonine kinases. This kinase contains 2 non-identical kinase catalytic domains and phosphorylates various substrates, including members of the mitogen-activated kinase (MAPK) signalling pathway. The activity of this protein has been implicated in controlling cell growth and differentiation. Alternate transcriptional splice variants, encoding different isoforms, have been characterized. [provided by RefSeq
  • Other Designations:
  • ribosomal S6 kinase 3,ribosomal protein S6 kinase alpha 2,ribosomal protein S6 kinase, 90kD, polypeptide 2
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