UBE2C monoclonal antibody, clone 1F5D3
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More Files
- More Functions
Western Blot (Cell lysate)
Western blot analysis of Lane 1: Hela cell; Lane 2: Raji cell with UBE2C monoclonal antibody.
Western Blot (Transfected lysate)
Western blot analysis of Lane 1: HEK293 cell; Lane 2: UBE2C-hIgGFc transfected HEK293 cell with UBE2C monoclonal antibody.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Immunohistochemical staining of paraffin-embedded esophageal cancer tissues with UBE2C monoclonal antibody.
Enzyme-linked Immunoabsorbent Assay
ELISA analysis of UBE2C monoclonal antibody, clone 1F5D3.
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Specification
Product Description
Mouse monoclonal antibody raised against recombinant human UBE2C.
Immunogen
Recombinant protein corresponding to amino acid 1-179 of human UBE2C from E. coli.
Host
Mouse
Theoretical MW (kDa)
19.7
Reactivity
Human
Form
Liquid
Isotype
IgG2a
Recommend Usage
ELISA (1:10000)
Western Blot (1:500-1:2000)
Immunohistochemistry (1:200-1:1000)
The optimal working dilution should be determined by the end user.Storage Buffer
In PBS (0.05% sodium azide).
Storage Instruction
Store at 4°C. For long term storage store at -20°C.
Aliquot to avoid repeated freezing and thawing.Note
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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Applications
Western Blot (Cell lysate)
Western blot analysis of Lane 1: Hela cell; Lane 2: Raji cell with UBE2C monoclonal antibody.Western Blot (Transfected lysate)
Western blot analysis of Lane 1: HEK293 cell; Lane 2: UBE2C-hIgGFc transfected HEK293 cell with UBE2C monoclonal antibody.Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Immunohistochemical staining of paraffin-embedded esophageal cancer tissues with UBE2C monoclonal antibody.Enzyme-linked Immunoabsorbent Assay
ELISA analysis of UBE2C monoclonal antibody, clone 1F5D3. -
Gene Info — UBE2C
Entrez GeneID
11065Gene Name
UBE2C
Gene Alias
UBCH10, dJ447F3.2
Gene Description
ubiquitin-conjugating enzyme E2C
Omim ID
605574Gene Ontology
HyperlinkGene Summary
The modification of proteins with ubiquitin is an important cellular mechanism for targeting abnormal or short-lived proteins for degradation. Ubiquitination involves at least three classes of enzymes: ubiquitin-activating enzymes, or E1s, ubiquitin-conjugating enzymes, or E2s, and ubiquitin-protein ligases, or E3s. This gene encodes a member of the E2 ubiquitin-conjugating enzyme family. This enzyme is required for the destruction of mitotic cyclins and for cell cycle progression. Multiple transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq
Other Designations
OTTHUMP00000031653|OTTHUMP00000031655|cyclin-selective ubiquitin carrier protein|mitotic-specific ubiquitin-conjugating enzyme|ubiquitin carrier protein E2-C|ubiquitin-protein ligase C
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Interactome
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Pathway
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Disease
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