CSNK2B monoclonal antibody, clone 2F12F3
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More Files
- More Functions
Western Blot (Cell lysate)
Western blot analysis of Lane 1: Hela cell; Lane 2: Jurkat cell; Lane 3: K562 cell; Lane 4: HepG2 cell; Lane 5: C6 cell; Lane 6: SK-N-SH cell; Lane 7: NTERA-2 cell; Lane 8: MCF-7 cell; Lane 9: NIH/3T3 cell with CSNK2B monoclonal antibody.
Western Blot (Transfected lysate)
Western blot analysis of Lane 1: HEK293 cell; Lane 2: CSNK2B-hIgGFc transfected HEK293 cell with CSNK2B monoclonal antibody.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Immunohistochemical staining of paraffin-embedded colon cancer tissues with CSNK2B monoclonal antibody.
Immunocytochemistry
Immunocytochemical staining of MCF-7 cells with CSNK2B monoclonal antibody (green). DRAQ5 fluorescent DNA dye (blue). Actin filaments labeled with Alexa Fluor-555 phalloidin (red).
Enzyme-linked Immunoabsorbent Assay
ELISA analysis of CSNK2B monoclonal antibody, clone 2F12F3.
Flow Cytometry
Flow cytometric analysis of Hela cells with CSNK2B monoclonal antibody (green) and negative control (red).
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Specification
Product Description
Mouse monoclonal antibody raised against recombinant human CSNK2B.
Immunogen
Recombinant protein corresponding to amino acid 1-215 of human CSNK2B from E. coli.
Host
Mouse
Theoretical MW (kDa)
25
Reactivity
Human
Form
Liquid
Isotype
IgG1
Recommend Usage
ELISA (1:10000)
Western Blot (1:500-1:2000)
Immunohistochemistry (1:200-1:1000)
Immunocytochemistry (1:200-1:1000)
Flow Cytometry (1:200-1:400)
The optimal working dilution should be determined by the end user.Storage Buffer
In PBS (0.05% sodium azide).
Storage Instruction
Store at 4°C. For long term storage store at -20°C.
Aliquot to avoid repeated freezing and thawing.Note
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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Applications
Western Blot (Cell lysate)
Western blot analysis of Lane 1: Hela cell; Lane 2: Jurkat cell; Lane 3: K562 cell; Lane 4: HepG2 cell; Lane 5: C6 cell; Lane 6: SK-N-SH cell; Lane 7: NTERA-2 cell; Lane 8: MCF-7 cell; Lane 9: NIH/3T3 cell with CSNK2B monoclonal antibody.Western Blot (Transfected lysate)
Western blot analysis of Lane 1: HEK293 cell; Lane 2: CSNK2B-hIgGFc transfected HEK293 cell with CSNK2B monoclonal antibody.Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Immunohistochemical staining of paraffin-embedded colon cancer tissues with CSNK2B monoclonal antibody.Immunocytochemistry
Immunocytochemical staining of MCF-7 cells with CSNK2B monoclonal antibody (green). DRAQ5 fluorescent DNA dye (blue). Actin filaments labeled with Alexa Fluor-555 phalloidin (red).Enzyme-linked Immunoabsorbent Assay
ELISA analysis of CSNK2B monoclonal antibody, clone 2F12F3.Flow Cytometry
Flow cytometric analysis of Hela cells with CSNK2B monoclonal antibody (green) and negative control (red). -
Gene Info — CSNK2B
Entrez GeneID
1460Gene Name
CSNK2B
Gene Alias
CK2B, CK2N, CSK2B, G5A, MGC138222, MGC138224
Gene Description
casein kinase 2, beta polypeptide
Omim ID
115441Gene Ontology
HyperlinkGene Summary
This gene encodes the beta subunit of casein kinase II, a ubiquitous protein kinase which regulates metabolic pathways, signal transduction, transcription, translation, and replication. The enzyme is composed of three subunits, alpha, alpha prime and beta, which form a tetrameric holoenzyme. The alpha and alpha prime subunits are catalytic, while the beta subunit serves regulatory functions. The enzyme localizes to the endoplasmic reticulum and the Golgi apparatus. [provided by RefSeq
Other Designations
Casein kinase II beta subunit|OTTHUMP00000029060|OTTHUMP00000029063|OTTHUMP00000029064|alternative name: G5a, phosvitin|phosvitin
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Interactome
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Pathway
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Disease
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