SHC1 monoclonal antibody, clone 2F7C7
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More Files
- More Functions
Western Blot (Cell lysate)
Western blot analysis using SHC1 monoclonal antibody against NIH/3T3 cell lysate.
Western Blot (Transfected lysate)
Western blot analysis of Lane 1: HEK293 cell; Lane 2: SHC1-hIgGFc transfected HEK293 cell with SHC1 monoclonal antibody.
Immunocytochemistry
Immunocytochemical staining of A431 cells with SHC1 monoclonal antibody (green). DRAQ5 fluorescent DNA dye (blue). Actin filaments labeled with Alexa Fluor-555 phalloidin (red).
Enzyme-linked Immunoabsorbent Assay
ELISA analysis of SHC1 monoclonal antibody, clone 2F7C7.
Flow Cytometry
Flow cytometric analysis of NIH/3T3 cells with SHC1 monoclonal antibody (green) and negative control (red).
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Specification
Product Description
Mouse monoclonal antibody raised against recombinant human SHC1.
Immunogen
Recombinant protein corresponding to amino acid 385-495 of human SHC1 from E. coli.
Host
Mouse
Theoretical MW (kDa)
62.8
Reactivity
Human, Mouse
Form
Liquid
Isotype
IgG1
Recommend Usage
ELISA (1:10000)
Western Blot (1:500-1:2000)
Immunocytochemistry (1:200-1:1000)
Flow Cytometry (1:200-1:400)
The optimal working dilution should be determined by the end user.Storage Buffer
In PBS (0.05% sodium azide).
Storage Instruction
Store at 4°C. For long term storage store at -20°C.
Aliquot to avoid repeated freezing and thawing.Note
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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Applications
Western Blot (Cell lysate)
Western blot analysis using SHC1 monoclonal antibody against NIH/3T3 cell lysate.Western Blot (Transfected lysate)
Western blot analysis of Lane 1: HEK293 cell; Lane 2: SHC1-hIgGFc transfected HEK293 cell with SHC1 monoclonal antibody.Immunocytochemistry
Immunocytochemical staining of A431 cells with SHC1 monoclonal antibody (green). DRAQ5 fluorescent DNA dye (blue). Actin filaments labeled with Alexa Fluor-555 phalloidin (red).Enzyme-linked Immunoabsorbent Assay
ELISA analysis of SHC1 monoclonal antibody, clone 2F7C7.Flow Cytometry
Flow cytometric analysis of NIH/3T3 cells with SHC1 monoclonal antibody (green) and negative control (red). -
Gene Info — SHC1
Entrez GeneID
6464Gene Name
SHC1
Gene Alias
FLJ26504, SHC, SHCA
Gene Description
SHC (Src homology 2 domain containing) transforming protein 1
Omim ID
600560Gene Ontology
HyperlinkOther Designations
OTTHUMP00000035409|OTTHUMP00000035471|SHC (Src homology 2 domain-containing) transforming protein 1|SHC-transforming protein 1
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Interactome
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Pathway
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Disease
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