PPP1CB monoclonal antibody, clone 8A7C7
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More Files
- More Functions
Western Blot (Cell lysate)
Western blot analysis of Lane 1: Jurkat cell; Lane 2: A431cell; Lane 3: Hela cell; Lane 4: HepG2 cell; Lane 5: HEK293 cell; Lane 6: MCF-7 cell with PPP1CB monoclonal antibody.
Western Blot (Transfected lysate)
Western blot analysis of Lane 1: HEK293 cell; Lane 2: PPP1CB-hIgGFc transfected HEK293 cell with PPP1CB monoclonal antibody.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Immunohistochemical staining of paraffin-embedded rectal cancer tissues with PPP1CB monoclonal antibody.
Immunocytochemistry
Immunocytochemical staining of MCF-7 cells with PPP1CB monoclonal antibody (green). DRAQ5 fluorescent DNA dye (blue). Actin filaments labeled with Alexa Fluor-555 phalloidin (red).
Enzyme-linked Immunoabsorbent Assay
ELISA analysis of PPP1CB monoclonal antibody, clone 8A7C7.
Flow Cytometry
Flow cytometric analysis of Jurkat cells with PPP1CB monoclonal antibody (green) and negative control (red).
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Specification
Product Description
Mouse monoclonal antibody raised against recombinant human PPP1CB.
Immunogen
Recombinant protein corresponding to amino acid 174-327 of human PPP1CB from E. coli.
Host
Mouse
Theoretical MW (kDa)
37.2
Reactivity
Human
Form
Liquid
Isotype
IgG1
Recommend Usage
ELISA (1:10000)
Western Blot (1:500-1:2000)
Immunohistochemistry (1:200-1:1000)
Immunocytochemistry (1:200-1:1000)
Flow Cytometry (1:200-1:400)
The optimal working dilution should be determined by the end user.Storage Buffer
In PBS (0.05% sodium azide).
Storage Instruction
Store at 4°C. For long term storage store at -20°C.
Aliquot to avoid repeated freezing and thawing.Note
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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Applications
Western Blot (Cell lysate)
Western blot analysis of Lane 1: Jurkat cell; Lane 2: A431cell; Lane 3: Hela cell; Lane 4: HepG2 cell; Lane 5: HEK293 cell; Lane 6: MCF-7 cell with PPP1CB monoclonal antibody.Western Blot (Transfected lysate)
Western blot analysis of Lane 1: HEK293 cell; Lane 2: PPP1CB-hIgGFc transfected HEK293 cell with PPP1CB monoclonal antibody.Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Immunohistochemical staining of paraffin-embedded rectal cancer tissues with PPP1CB monoclonal antibody.Immunocytochemistry
Immunocytochemical staining of MCF-7 cells with PPP1CB monoclonal antibody (green). DRAQ5 fluorescent DNA dye (blue). Actin filaments labeled with Alexa Fluor-555 phalloidin (red).Enzyme-linked Immunoabsorbent Assay
ELISA analysis of PPP1CB monoclonal antibody, clone 8A7C7.Flow Cytometry
Flow cytometric analysis of Jurkat cells with PPP1CB monoclonal antibody (green) and negative control (red). -
Gene Info — PPP1CB
Entrez GeneID
5500Gene Name
PPP1CB
Gene Alias
MGC3672, PP-1B, PPP1CD
Gene Description
protein phosphatase 1, catalytic subunit, beta isoform
Omim ID
600590Gene Ontology
HyperlinkGene Summary
The protein encoded by this gene is one of the three catalytic subunits of protein phosphatase 1 (PP1). PP1 is a serine/threonine specific protein phosphatase known to be involved in the regulation of a variety of cellular processes, such as cell division, glycogen metabolism, muscle contractility, protein synthesis, and HIV-1 viral transcription. Mouse studies suggest that PP1 functions as a suppressor of learning and memory. Two alternatively spliced transcript variants encoding distinct isoforms have been observed. [provided by RefSeq
Other Designations
protein phosphatase 1, catalytic subunit, beta|protein phosphatase 1, catalytic subunit, delta isoform|protein phosphatase 1-beta|protein phosphatase 1-delta|serine/threonine protein phosphatase PP1-beta catalytic subunit
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Interactome
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Pathway
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Disease
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