CAV2 monoclonal antibody, clone 5E9E2
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More Files
- More Functions
Western Blot (Cell lysate)
Western blot analysis of Lane 1: A549 cell; Lane 2: 3T3-L1 cell; Lane 3:A431 cell with CAV2 monoclonal antibody.
Western Blot (Transfected lysate)
Western blot analysis of Lane 1: HEK293 cell; Lane 2: CAV2-hIgGFc transfected HEK293 cell with CAV2 monoclonal antibody.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Immunohistochemical staining of paraffin-embedded endometrial cancer tissues with CAV2 monoclonal antibody.
Enzyme-linked Immunoabsorbent Assay
ELISA analysis of CAV2 monoclonal antibody, clone 5E9E2.
Flow Cytometry
Flow cytometric analysis of A549 cells with CAV2 monoclonal antibody (green) and negative control (red).
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Specification
Product Description
Mouse monoclonal antibody raised against recombinant human CAV2.
Immunogen
Recombinant protein corresponding to amino acid 1-86 of human CAV2 from E. coli.
Host
Mouse
Theoretical MW (kDa)
18.3
Reactivity
Human
Form
Liquid
Isotype
IgG1
Recommend Usage
ELISA (1:10000)
Western Blot (1:500-1:2000)
Immunohistochemistry (1:200-1:1000)
Flow Cytometry (1:200-1:400)
The optimal working dilution should be determined by the end user.Storage Buffer
In PBS (0.05% sodium azide).
Storage Instruction
Store at 4°C. For long term storage store at -20°C.
Aliquot to avoid repeated freezing and thawing.Note
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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Applications
Western Blot (Cell lysate)
Western blot analysis of Lane 1: A549 cell; Lane 2: 3T3-L1 cell; Lane 3:A431 cell with CAV2 monoclonal antibody.Western Blot (Transfected lysate)
Western blot analysis of Lane 1: HEK293 cell; Lane 2: CAV2-hIgGFc transfected HEK293 cell with CAV2 monoclonal antibody.Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Immunohistochemical staining of paraffin-embedded endometrial cancer tissues with CAV2 monoclonal antibody.Enzyme-linked Immunoabsorbent Assay
ELISA analysis of CAV2 monoclonal antibody, clone 5E9E2.Flow Cytometry
Flow cytometric analysis of A549 cells with CAV2 monoclonal antibody (green) and negative control (red). -
Gene Info — CAV2
Entrez GeneID
858Gene Name
CAV2
Gene Alias
CAV, MGC12294
Gene Description
caveolin 2
Omim ID
601048Gene Ontology
HyperlinkGene Summary
The protein encoded by this gene is a major component of the inner surface of caveolae, small invaginations of the plasma membrane, and is involved in essential cellular functions, including signal transduction, lipid metabolism, cellular growth control and apoptosis. This protein may function as a tumor suppressor. CAV1 and CAV2 are located next to each other on chromosome 7 and express colocalizing proteins that form a stable hetero-oligomeric complex. Two transcript variants encoding distinct isoforms have been identified for this gene. By using alternative initiation codons in the same reading frame, two isoforms (alpha and beta) are encoded by one transcript. [provided by RefSeq
Other Designations
caveolae protein, 20-kD|caveolin 2 isoform a and b
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Interactome
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Pathway
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Disease
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