BAD monoclonal antibody, clone 1G5B3
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More Files
- More Functions
Western Blot (Cell lysate)
Western blot analysis of Lane 1: MCF-7 cell; Lane 2: HEK293 cell with BAD monoclonal antibody.
Western Blot (Transfected lysate)
Western blot analysis of Lane 1: HEK293 cell; Lane 2: BAD-hIgGFc transfected HEK293 cell with BAD monoclonal antibody.
Enzyme-linked Immunoabsorbent Assay
ELISA analysis of BAD monoclonal antibody, clone 1G5B3.
Flow Cytometry
Flow cytometric analysis of MCF-7 cells with BAD monoclonal antibody (green) and negative control (red).
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Specification
Product Description
Mouse monoclonal antibody raised against recombinant human BAD.
Immunogen
Recombinant protein corresponding to amino acid 1-168 of human BAD from E. coli.
Host
Mouse
Theoretical MW (kDa)
18.4
Reactivity
Human
Form
Liquid
Isotype
IgG1
Recommend Usage
ELISA (1:10000)
Western Blot (1:500-1:2000)
Flow Cytometry (1:200-1:400)
The optimal working dilution should be determined by the end user.Storage Buffer
In PBS (0.05% sodium azide).
Storage Instruction
Store at 4°C. For long term storage store at -20°C.
Aliquot to avoid repeated freezing and thawing.Note
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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Applications
Western Blot (Cell lysate)
Western blot analysis of Lane 1: MCF-7 cell; Lane 2: HEK293 cell with BAD monoclonal antibody.Western Blot (Transfected lysate)
Western blot analysis of Lane 1: HEK293 cell; Lane 2: BAD-hIgGFc transfected HEK293 cell with BAD monoclonal antibody.Enzyme-linked Immunoabsorbent Assay
ELISA analysis of BAD monoclonal antibody, clone 1G5B3.Flow Cytometry
Flow cytometric analysis of MCF-7 cells with BAD monoclonal antibody (green) and negative control (red). -
Gene Info — BAD
Entrez GeneID
572Gene Name
BAD
Gene Alias
BBC2, BCL2L8
Gene Description
BCL2-associated agonist of cell death
Omim ID
603167Gene Ontology
HyperlinkGene Summary
The protein encoded by this gene is a member of the BCL-2 family. BCL-2 family members are known to be regulators of programmed cell death. This protein positively regulates cell apoptosis by forming heterodimers with BCL-xL and BCL-2, and reversing their death repressor activity. Proapoptotic activity of this protein is regulated through its phosphorylation. Protein kinases AKT and MAP kinase, as well as protein phosphatase calcineurin were found to be involved in the regulation of this protein. Alternative splicing of this gene results in two transcript variants which encode the same isoform. [provided by RefSeq
Other Designations
BCL-X/BCL-2 binding protein|BCL2-antagonist of cell death protein|BCL2-binding component 6|BCL2-binding protein
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Interactome
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Pathway
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Disease
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