DAPK3 monoclonal antibody, clone 4H4C8
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More Files
- More Functions
Western Blot (Cell lysate)
Western blot analysis of Lane 1: A431 cell; Lane 2: K562 cell with DAPK3 monoclonal antibody.
Western Blot (Transfected lysate)
Western blot analysis of Lane 1: HEK293 cell; Lane 2: DAPK3-hIgGFc transfected HEK293 cell with DAPK3 monoclonal antibody.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Immunohistochemical staining of paraffin-embedded rectal cancer tissues with DAPK3 monoclonal antibody.
Enzyme-linked Immunoabsorbent Assay
ELISA analysis of DAPK3 monoclonal antibody, clone 4H4C8.
Flow Cytometry
Flow cytometric analysis of A431 cells with DAPK3 monoclonal antibody (green) and negative control (red).
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Specification
Product Description
Mouse monoclonal antibody raised against recombinant human DAPK3.
Immunogen
Recombinant protein corresponding to amino acid 28-161 of human DAPK3 from E. coli.
Host
Mouse
Theoretical MW (kDa)
52.5
Reactivity
Human
Form
Liquid
Isotype
IgG1
Recommend Usage
ELISA (1:10000)
Western Blot (1:500-1:2000)
Immunohistochemistry (1:200-1:1000)
Flow Cytometry (1:200-1:400)
The optimal working dilution should be determined by the end user.Storage Buffer
In PBS (0.05% sodium azide).
Storage Instruction
Store at 4°C. For long term storage store at -20°C.
Aliquot to avoid repeated freezing and thawing.Note
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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Applications
Western Blot (Cell lysate)
Western blot analysis of Lane 1: A431 cell; Lane 2: K562 cell with DAPK3 monoclonal antibody.Western Blot (Transfected lysate)
Western blot analysis of Lane 1: HEK293 cell; Lane 2: DAPK3-hIgGFc transfected HEK293 cell with DAPK3 monoclonal antibody.Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Immunohistochemical staining of paraffin-embedded rectal cancer tissues with DAPK3 monoclonal antibody.Enzyme-linked Immunoabsorbent Assay
ELISA analysis of DAPK3 monoclonal antibody, clone 4H4C8.Flow Cytometry
Flow cytometric analysis of A431 cells with DAPK3 monoclonal antibody (green) and negative control (red). -
Gene Info — DAPK3
Entrez GeneID
1613Gene Name
DAPK3
Gene Alias
FLJ36473, ZIP, ZIPK
Gene Description
death-associated protein kinase 3
Omim ID
603289Gene Ontology
HyperlinkGene Summary
Death-associated protein kinase 3 (DAPK3) induces morphological changes in apoptosis when overexpressed in mammalian cells. These results suggest that DAPK3 may play a role in the induction of apoptosis. [provided by RefSeq
Other Designations
ZIP kinase isoform
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Interactome
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Pathway
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