Acrolein monoclonal antibody, clone 2H2
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More Files
- More Functions
Immunocytochemistry
Immunocytochemical staining of HEK293 with Acrolein monoclonal antibody, clone 2H2 (Cat # MAB16478). (A, B) DAPI (blue) nuclear stain, (C, D) Phalloidin Alex Fluor 633 F-Actin stain, (E, F) Acrolein Antibody and (G, H) Composite. (A, C, E, G) Untreated and (B, D, F, H) Cells cultured overnight with 50 uM H2O2.
Flow Cytometry
Flow cytometric analysis of SH-SY5Y with Acrolein monoclonal antibody, clone 2H2 (Cat # MAB16478).
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Specification
Product Description
Mouse monoclonal antibody raised against synthetic peptide of Acrolein.
Immunogen
Synthetic Acrolein modified Keyhole Limpet Hemocyanin (KLH).
Host
Mouse
Reactivity
Human
Form
Liquid
Purification
Protein G purification
Isotype
IgG1
Recommend Usage
ELISA (1:1000)
Flow Cytometry
Immunocytochemistry (1:50)
Immunofluorescence (1:50)
Western Blot (1:1000)
The optimal working dilution should be determined by the end user.Storage Buffer
In PBS, pH 7.4 (50% glycerol, 0.09% sodium azide).
Storage Instruction
Store at -20°C.
Note
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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Applications
Western Blot
Immunocytochemistry
Immunocytochemical staining of HEK293 with Acrolein monoclonal antibody, clone 2H2 (Cat # MAB16478). (A, B) DAPI (blue) nuclear stain, (C, D) Phalloidin Alex Fluor 633 F-Actin stain, (E, F) Acrolein Antibody and (G, H) Composite. (A, C, E, G) Untreated and (B, D, F, H) Cells cultured overnight with 50 uM H2O2.Immunofluorescence
Enzyme-linked Immunoabsorbent Assay
Flow Cytometry
Flow cytometric analysis of SH-SY5Y with Acrolein monoclonal antibody, clone 2H2 (Cat # MAB16478).
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