CRYAB monoclonal antibody, clone 6D11
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More Files
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Specification
Product Description
Mouse monoclonal antibody raised against full length recombinant human CRYAB.
Immunogen
Recombinant protein corresponding to full length human CRYAB from HEK293T cell.
Host
Mouse
Reactivity
Human
Form
Liquid
Purification
Protein A/G purification
Isotype
IgG1
Recommend Usage
Flow Cytometry (1:100)
Immunofluorescence (1:50-1:100)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) (10 ug/mL)
Immunoprecipitation
Western Blot (1:500-1:1000)
The optimal working dilution should be determined by the end user.Storage Buffer
In PBS, pH 7.3 (50% glycerol, 1% BSA, 0.02% sodium azide).
Storage Instruction
Store at -20°C.
Aliquot to avoid repeated freezing and thawing.Note
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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Applications
Western Blot (Transfected lysate)
Western Blot analysis of HEK293T cells (Lane 1: transfected with pCMV6-ENTRY control and Lane 2: transfected with pCMV6-ENTRY CRYAB cDNA) with CRYAB monoclonal antibody, clone 6D11 (Cat # MAB16409).Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Immunohistochemical staining (Formalin-fixed paraffin-embedded sections) of human heart with CRYAB monoclonal antibody, clone 6D11 (Cat # MAB16409).Immunofluorescence
Immunofluorescent staining of COS7 cells transiently transfected by pCMV6-ENTRY CRYAB with CRYAB monoclonal antibody, clone 6D11 (Cat # MAB16409).Immunoprecipitation
Flow Cytometry
Flow cytometric analysis of HEK293T cells transfected with either overexpress plasmid (Red) or empty vector control plasmid (Blue) using CRYAB monoclonal antibody, clone 6D11 (Cat # MAB16409). -
Gene Info — CRYAB
Entrez GeneID
1410Protein Accession#
P02511Gene Name
CRYAB
Gene Alias
CRYA2, CTPP2, HSPB5
Gene Description
crystallin, alpha B
Gene Ontology
HyperlinkGene Summary
Crystallins are separated into two classes: taxon-specific, or enzyme, and ubiquitous. The latter class constitutes the major proteins of vertebrate eye lens and maintains the transparency and refractive index of the lens. Since lens central fiber cells lose their nuclei during development, these crystallins are made and then retained throughout life, making them extremely stable proteins. Mammalian lens crystallins are divided into alpha, beta, and gamma families; beta and gamma crystallins are also considered as a superfamily. Alpha and beta families are further divided into acidic and basic groups. Seven protein regions exist in crystallins: four homologous motifs, a connecting peptide, and N- and C-terminal extensions. Alpha crystallins are composed of two gene products: alpha-A and alpha-B, for acidic and basic, respectively. Alpha crystallins can be induced by heat shock and are members of the small heat shock protein (sHSP also known as the HSP20) family. They act as molecular chaperones although they do not renature proteins and release them in the fashion of a true chaperone; instead they hold them in large soluble aggregates. Post-translational modifications decrease the ability to chaperone. These heterogeneous aggregates consist of 30-40 subunits; the alpha-A and alpha-B subunits have a 3:1 ratio, respectively. Two additional functions of alpha crystallins are an autokinase activity and participation in the intracellular architecture. Alpha-A and alpha-B gene products are differentially expressed; alpha-A is preferentially restricted to the lens and alpha-B is expressed widely in many tissues and organs. Elevated expression of alpha-B crystallin occurs in many neurological diseases; a missense mutation cosegregated in a family with a desmin-related myopathy. [provided by RefSeq
Other Designations
alpha crystallin B chain|heat-shock 20 kD like-protein
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