SF3B4 monoclonal antibody, clone 3A1
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More Files
- More Functions
Western Blot (Cell lysate)
Western Blot analysis of HeLa cell lysate with SF3B4 monoclonal antibody, clone 3A1 (Cat # MAB16402).
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Immunohistochemical staining (Formalin-fixed paraffin-embedded sections) of human tonsil with SF3B4 monoclonal antibody, clone 3A1 (Cat # MAB16402).
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Specification
Product Description
Mouse monoclonal antibody raised against full length recombinant human SF3B4.
Immunogen
Recombinant protein corresponding to full length human SF3B4 from E. coli.
Host
Mouse
Reactivity
Human
Form
Liquid
Purification
Affinity purification
Isotype
IgG2b, kappa
Recommend Usage
Immunocytochemistry (1:1000)
Immunofluorescence (1:1000)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) (10 ug/mL)
Western Blot (1:1000)
The optimal working dilution should be determined by the end user.Storage Buffer
In PBS (10 mM sodium azide).
Storage Instruction
Store at 4°C. For long term storage store at -20°C.
Aliquot to avoid repeated freezing and thawing.Note
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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Applications
Western Blot (Cell lysate)
Western Blot analysis of HeLa cell lysate with SF3B4 monoclonal antibody, clone 3A1 (Cat # MAB16402).Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Immunohistochemical staining (Formalin-fixed paraffin-embedded sections) of human tonsil with SF3B4 monoclonal antibody, clone 3A1 (Cat # MAB16402).Immunocytochemistry
Immunofluorescence
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Gene Info — SF3B4
Entrez GeneID
10262Protein Accession#
Q15427Gene Name
SF3B4
Gene Alias
MGC10828, SAP49, SF3b49
Gene Description
splicing factor 3b, subunit 4, 49kDa
Omim ID
605593Gene Ontology
HyperlinkGene Summary
This gene encodes one of four subunits of the splicing factor 3B. The protein encoded by this gene cross-links to a region in the pre-mRNA immediately upstream of the branchpoint sequence in pre-mRNA in the prespliceosomal complex A. It also may be involved in the assembly of the B, C and E spliceosomal complexes. In addition to RNA-binding activity, this protein interacts directly and highly specifically with subunit 2 of the splicing factor 3B. This protein contains two N-terminal RNA-recognition motifs (RRMs), consistent with the observation that it binds directly to pre-mRNA. [provided by RefSeq
Other Designations
OTTHUMP00000014064|pre-mRNA splicing factor SF3b 49 kDa subunit|spliceosomal protein|spliceosome-associated protein (U2 snRNP)|splicing factor 3b, subunit 4
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