STAT5A monoclonal antibody, clone 9F7
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More Files
- More Functions
Western Blot (Transfected lysate)
Western Blot analysis of HEK293T cells (Lane 1: transfected with pCMV6-ENTRY control and Lane 2: transfected with pCMV6-ENTRY STAT5A cDNA) with STAT5A monoclonal antibody, clone 9F7 (Cat # MAB16377).
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Immunohistochemical staining (Formalin-fixed paraffin-embedded sections) of human testis with STAT5A monoclonal antibody, clone 9F7 (Cat # MAB16377).
Immunofluorescence
Immunofluorescent staining of COS7 cells transiently transfected by pCMV6-ENTRY STAT5A with STAT5A monoclonal antibody, clone 9F7 (Cat # MAB16377).
Flow Cytometry
Flow cytometric analysis of HeLa cells with STAT5A monoclonal antibody, clone 9F7 (Cat # MAB16377) (Red).
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Specification
Product Description
Mouse monoclonal antibody raised against full length recombinant human STAT5A.
Immunogen
Recombinant protein corresponding to full length human STAT5A from HEK293T cell.
Host
Mouse
Reactivity
Human
Form
Liquid
Purification
Protein A/G purification
Isotype
IgG1
Recommend Usage
Flow Cytometry (1:100)
Immunofluorescence (1:100)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) (10 ug/mL)
Western Blot (1:500-1:2000)
The optimal working dilution should be determined by the end user.Storage Buffer
In PBS, pH 7.3 (50% glycerol, 1% BSA, 0.02% sodium azide).
Storage Instruction
Store at -20°C.
Aliquot to avoid repeated freezing and thawing.Note
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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Applications
Western Blot (Transfected lysate)
Western Blot analysis of HEK293T cells (Lane 1: transfected with pCMV6-ENTRY control and Lane 2: transfected with pCMV6-ENTRY STAT5A cDNA) with STAT5A monoclonal antibody, clone 9F7 (Cat # MAB16377).Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Immunohistochemical staining (Formalin-fixed paraffin-embedded sections) of human testis with STAT5A monoclonal antibody, clone 9F7 (Cat # MAB16377).Immunofluorescence
Immunofluorescent staining of COS7 cells transiently transfected by pCMV6-ENTRY STAT5A with STAT5A monoclonal antibody, clone 9F7 (Cat # MAB16377).Flow Cytometry
Flow cytometric analysis of HeLa cells with STAT5A monoclonal antibody, clone 9F7 (Cat # MAB16377) (Red). -
Gene Info — STAT5A
Entrez GeneID
6776Protein Accession#
P42229Gene Name
STAT5A
Gene Alias
MGF, STAT5
Gene Description
signal transducer and activator of transcription 5A
Omim ID
601511Gene Ontology
HyperlinkGene Summary
The protein encoded by this gene is a member of the STAT family of transcription factors. In response to cytokines and growth factors, STAT family members are phosphorylated by the receptor associated kinases, and then form homo- or heterodimers that translocate to the cell nucleus where they act as transcription activators. This protein is activated by, and mediates the responses of many cell ligands, such as IL2, IL3, IL7 GM-CSF, erythropoietin, thrombopoietin, and different growth hormones. Activation of this protein in myeloma and lymphoma associated with a TEL/JAK2 gene fusion is independent of cell stimulus and has been shown to be essential for the tumorigenesis. The mouse counterpart of this gene is found to induce the expression of BCL2L1/BCL-X(L), which suggests the antiapoptotic function of this gene in cells. [provided by RefSeq
Other Designations
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