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Last updated: 2019/9/15

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FGF2 monoclonal antibody, clone 3D9 

  • Catalog # : MAB16376
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  • Specification
  • Product Description:
  • Mouse monoclonal antibody raised against partial recombinant human FGF2.
  • Immunogen:
  • Recombinant protein corresponding to amino acids 10-155 of human FGF2 from E. coli.
  • Host:
  • Mouse
  • Reactivity:
  • Human
  • Form:
  • Liquid
  • Purification:
  • Protein A/G purification
  • Isotype:
  • IgG2b
  • Recommend Usage:
  • Immunofluorescence (1:100)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) (10 ug/mL)
    Western Blot (1:1000)
    The optimal working dilution should be determined by the end user.
  • Storage Buffer:
  • In PBS, pH 7.3 (50% glycerol, 1% BSA, 0.02% sodium azide).
  • Storage Instruction:
  • Store at -20°C.
    Aliquot to avoid repeated freezing and thawing.
  • Note:
  • This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
  • Applications
  • Western Blot (Transfected lysate)
  • Western Blot (Transfected lysate)
  • Western Blot analysis of HEK293T cells (Lane 1: transfected with pCMV6-ENTRY control and Lane 2: transfected with pCMV6-ENTRY FGF2 cDNA) with FGF2 monoclonal antibody, clone 3D9 (Cat # MAB16376).
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
  • Immunohistochemical staining (Formalin-fixed paraffin-embedded sections) of human cortex with FGF2 monoclonal antibody, clone 3D9 (Cat # MAB16376).
  • Immunofluorescence
  • Immunofluorescence
  • Immunofluorescent staining of COS7 cells transiently transfected by pCMV6-ENTRY FGF2 with FGF2 monoclonal antibody, clone 3D9 (Cat # MAB16376).
  • Application Image
  • Western Blot (Transfected lysate)
  • Western Blot (Transfected lysate)
  • enlarge
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
  • enlarge
  • Gene Information
  • Entrez GeneID:
  • 2247
  • Gene Name:
  • FGF2
  • Gene Alias:
  • BFGF,FGFB,HBGF-2
  • Gene Description:
  • fibroblast growth factor 2 (basic)
  • Gene Summary:
  • The protein encoded by this gene is a member of the fibroblast growth factor (FGF) family. FGF family members bind heparin and possess broad mitogenic and angiogenic activities. This protein has been implicated in diverse biological processes, such as limb and nervous system development, wound healing, and tumor growth. The mRNA for this gene contains multiple polyadenylation sites, and is alternatively translated from non-AUG (CUG) and AUG initiation codons, resulting in five different isoforms with distinct properties. The CUG-initiated isoforms are localized in the nucleus and are responsible for the intracrine effect, whereas, the AUG-initiated form is mostly cytosolic and is responsible for the paracrine and autocrine effects of this FGF. [provided by RefSeq
  • Other Designations:
  • basic fibroblast growth factor bFGF,fibroblast growth factor 2,heparin-binding growth factor 2,prostatropin
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