Mouse monoclonal antibody raised against partial recombinant human FGF2.
Immunogen:
Recombinant protein corresponding to amino acids 10-155 of human FGF2 from E. coli.
Host:
Mouse
Reactivity:
Human
Form:
Liquid
Purification:
Protein A/G purification
Isotype:
IgG2b
Recommend Usage:
Immunofluorescence (1:100) Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) (10 ug/mL) Western Blot (1:1000) The optimal working dilution should be determined by the end user.
Western Blot analysis of HEK293T cells (Lane 1: transfected with pCMV6-ENTRY control and Lane 2: transfected with pCMV6-ENTRY FGF2 cDNA) with FGF2 monoclonal antibody, clone 3D9 (Cat # MAB16376).
The protein encoded by this gene is a member of the fibroblast growth factor (FGF) family. FGF family members bind heparin and possess broad mitogenic and angiogenic activities. This protein has been implicated in diverse biological processes, such as limb and nervous system development, wound healing, and tumor growth. The mRNA for this gene contains multiple polyadenylation sites, and is alternatively translated from non-AUG (CUG) and AUG initiation codons, resulting in five different isoforms with distinct properties. The CUG-initiated isoforms are localized in the nucleus and are responsible for the intracrine effect, whereas, the AUG-initiated form is mostly cytosolic and is responsible for the paracrine and autocrine effects of this FGF. [provided by RefSeq