MAPK1 monoclonal antibody, clone B20-U
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More Files
- More Functions
Western Blot (Tissue lysate)
MAPK1 monoclonal antibody, clone B20-U (Cat # MAB1636). Western blot of MAPK1 in rat brain crude lysate (100µg of protein loaded).
Immunofluorescence
Immunofluorescence staining of HEK293 cells with MAPK1 monoclonal antibody, clone B20-U (Cat # MAB1636) at 1:200 dilution.
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Specification
Product Description
Rabbit monoclonal antibody raised against synthetic peptide of MAPK1.
Immunogen
A synthetic peptide corresponding to C-terminus of human MAPK1.
Host
Rabbit
Reactivity
Human, Mouse, Rat
Form
Liquid
Isotype
IgG
Quality Control Testing
Antibody Reactive Against Synthetic Peptide.
Recommend Usage
Western Blot (1:5000)
ELISA (1:20000-1:100000)
The optimal working dilution should be determined by the end user.Storage Buffer
In 20 mM Tris-HCl, pH 8.0 (10 mg/mL BSA, 0.05% sodium azide)
Storage Instruction
Store at 4°C.
Note
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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Applications
Western Blot (Tissue lysate)
MAPK1 monoclonal antibody, clone B20-U (Cat # MAB1636). Western blot of MAPK1 in rat brain crude lysate (100µg of protein loaded).Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Immunohistochemistry (Frozen sections)
Immunofluorescence
Immunofluorescence staining of HEK293 cells with MAPK1 monoclonal antibody, clone B20-U (Cat # MAB1636) at 1:200 dilution.Immunoprecipitation
Enzyme-linked Immunoabsorbent Assay
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Gene Info — MAPK1
Entrez GeneID
5594Gene Name
MAPK1
Gene Alias
ERK, ERK2, ERT1, MAPK2, P42MAPK, PRKM1, PRKM2, p38, p40, p41, p41mapk
Gene Description
mitogen-activated protein kinase 1
Omim ID
176948Gene Ontology
HyperlinkGene Summary
The protein encoded by this gene is a member of the MAP kinase family. MAP kinases, also known as extracellular signal-regulated kinases (ERKs), act as an integration point for multiple biochemical signals, and are involved in a wide variety of cellular processes such as proliferation, differentiation, transcription regulation and development. The activation of this kinase requires its phosphorylation by upstream kinases. Upon activation, this kinase translocates to the nucleus of the stimulated cells, where it phosphorylates nuclear targets. Two alternatively spliced transcript variants encoding the same protein, but differing in the UTRs, have been reported for this gene. [provided by RefSeq
Other Designations
OTTHUMP00000174492|extracellular signal-regulated kinase 2|extracellular signal-regulated kinase-2|mitogen-activated protein kinase 2|protein tyrosine kinase ERK2
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Interactome
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Pathway
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Disease
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