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Last updated: 2017/12/10

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NCK1 monoclonal antibody, clone 11D10

  • Catalog # : MAB16226
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  • Specification
  • Product Description:
  • Mouse monoclonal antibody raised against recombinant of human NCK1.
  • Immunogen:
  • Recombinant His fusion protein corresponding to human NCK1 from E. coli.
  • Host:
  • Mouse
  • Reactivity:
  • Human
  • Form:
  • Liquid
  • Purification:
  • Ammonium sulfate precipitation
  • Isotype:
  • IgG1,k
  • Recommend Usage:
  • ELISA
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) (1:200)
    Western Blot (1:2000)
    The optimal working dilution should be determined by the end user.
  • Storage Buffer:
  • In HEPES, 150 mM NaCl (50% glycerol, 0.01% BSA, 0.03% sodium azide).
  • Storage Instruction:
  • Store at 4°C. For long term storage store at -20°C.
    Aliquot to avoid repeated freezing and thawing.
  • Note:
  • This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
  • Applications
  • Western Blot (Cell lysate)
  • Western Blot (Cell lysate)
  • Western Blot analysis of Lane 1: HeLa, Lane 2: RD, Lane 3: PC12 and Lane 4: NIH3T3 cell lysates with NCK1 monoclonal antibody, clone 11D10 (Cat # MAB16226).
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
  • Immunohistochemical staining (Formalin-fixed paraffin-embedded sections) of human tonsil with NCK1 monoclonal antibody, clone 11D10 (Cat # MAB16226).
  • ELISA
  • Application Image
  • Western Blot (Cell lysate)
  • Western Blot (Cell lysate)
  • enlarge
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
  • enlarge
  • ELISA
  • Gene Information
  • Entrez GeneID:
  • 4690
  • Gene Name:
  • NCK1
  • Gene Alias:
  • MGC12668,NCK,NCKalpha
  • Gene Description:
  • NCK adaptor protein 1
  • Gene Summary:
  • The protein encoded by this gene is one of the signaling and transforming proteins containing Src homology 2 and 3 (SH2 and SH3) domains. It is located in the cytoplasm and is an adaptor protein involved in transducing signals from receptor tyrosine kinases to downstream signal recipients such as RAS. [provided by RefSeq
  • Other Designations:
  • NCK tyrosine kinase,SH2/SH3 adaptor protein NCK-alpha,melanoma NCK protein,non-catalytic region of tyrosine kinase
  • Interactome
  • Interactome
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