NRAS monoclonal antibody, clone AT2G9
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More Files
- More Functions
Western Blot (Cell lysate)
Western Blot analysis of 293T cell lysate (Lane 1: 1:500, Lane 2: 1:1000 and Lane 3: 1:5000) with NRAS monoclonal antibody, clone AT2G9 (Cat # MAB16186).
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Immunohistochemical staining (Formalin-fixed paraffin-embedded sections) of human pancreas with NRAS monoclonal antibody, clone AT2G9 (Cat # MAB16186).
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Specification
Product Description
Mouse monoclonal antibody raised against recombinant human NRAS.
Immunogen
Recombinant protein corresponding to human NRAS.
Host
Mouse
Reactivity
Human
Form
Liquid
Purification
Protein G purification
Isotype
IgG2a, kappa
Recommend Usage
ELISA
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) (15 ug/mL)
Western Blot (1:500-1:5000)
The optimal working dilution should be determined by the end user.Storage Buffer
In PBS, pH 7.4 (0.09% sodium azide).
Storage Instruction
Store at 4°C. For long term storage store at -20°C.
Aliquot to avoid repeated freezing and thawing.Note
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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Applications
Western Blot (Cell lysate)
Western Blot analysis of 293T cell lysate (Lane 1: 1:500, Lane 2: 1:1000 and Lane 3: 1:5000) with NRAS monoclonal antibody, clone AT2G9 (Cat # MAB16186).Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Immunohistochemical staining (Formalin-fixed paraffin-embedded sections) of human pancreas with NRAS monoclonal antibody, clone AT2G9 (Cat # MAB16186).Enzyme-linked Immunoabsorbent Assay
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Gene Info — NRAS
Entrez GeneID
4893Protein Accession#
P01111Gene Name
NRAS
Gene Alias
ALPS4, N-ras, NRAS1
Gene Description
neuroblastoma RAS viral (v-ras) oncogene homolog
Gene Ontology
HyperlinkGene Summary
This is an N-ras oncogene encoding a membrane protein that shuttles between the Golgi apparatus and the plasma membrane. This shuttling is regulated through palmitoylation and depalmitoylation by the ZDHHC9-GOLGA7 complex. The encoded protein, which has intrinsic GTPase activity, is activated to a GTP-bound form by a GTPase activating protein and inactivated to a GDP-bound form by a guanine nucleotide-exchange factor. Defects in this gene are a cause of juvenile myelomonocytic leukemia (JMML). [provided by RefSeq
Other Designations
N-ras protein part 4|OTTHUMP00000013879|v-ras neuroblastoma RAS viral oncogene homolog
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Interactome
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Pathway
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Disease
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