PRKCZ monoclonal antibody, clone R14-K
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More Files
- More Functions
Western Blot
Western Blot analysis of Lane 1: PRKCZ recombinant protein, Lane 2: mouse brain crude lysate and Lane 3: rat brain crude lysate with PRKCZ monoclonal antibody, clone R14-K (Cat # MAB15905).
Immunocytochemistry
Immunocytochemical staining of HEK293 cells with PRKCZ monoclonal antibody, clone R14-K (Cat # MAB15905) at 1:200 dilution.
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Specification
Product Description
Rabbit monoclonal antibody raised against synthetic peptide of human PRKCZ.
Immunogen
A synthetic peptide corresponding to amino acids 220-231 at internal region of human PRKCZ.
Host
Rabbit
Reactivity
Human, Mouse, Rat
Form
Liquid
Purification
EVAC purification
Isotype
IgG
Recommend Usage
ELISA (1:20000-1:100000)
Immunocytochemistry
Immunoprecipitation
Western Blot (1:5000)
The optimal working dilution should be determined by the end user.Storage Buffer
In 20 mM Tris-HCl buffer, pH 8.0 (10 mg/mL BSA, 0.05% Sodium Azide).
Storage Instruction
Store at -20°C.
Aliquot to avoid repeated freezing and thawing.Note
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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Applications
Western Blot
Western Blot analysis of Lane 1: PRKCZ recombinant protein, Lane 2: mouse brain crude lysate and Lane 3: rat brain crude lysate with PRKCZ monoclonal antibody, clone R14-K (Cat # MAB15905).Immunocytochemistry
Immunocytochemical staining of HEK293 cells with PRKCZ monoclonal antibody, clone R14-K (Cat # MAB15905) at 1:200 dilution.Immunoprecipitation
Enzyme-linked Immunoabsorbent Assay
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Gene Info — PRKCZ
Entrez GeneID
5590Protein Accession#
Q05513Gene Name
PRKCZ
Gene Alias
PKC-ZETA, PKC2
Gene Description
protein kinase C, zeta
Omim ID
176982Gene Ontology
HyperlinkGene Summary
Protein kinase C (PKC) zeta is a member of the PKC family of serine/threonine kinases which are involved in a variety of cellular processes such as proliferation, differentiation and secretion. Unlike the classical PKC isoenzymes which are calcium-dependent, PKC zeta exhibits a kinase activity which is independent of calcium and diacylglycerol but not of phosphatidylserine. Furthermore, it is insensitive to typical PKC inhibitors and cannot be activated by phorbol ester. Unlike the classical PKC isoenzymes, it has only a single zinc finger module. These structural and biochemical properties indicate that the zeta subspecies is related to, but distinct from other isoenzymes of PKC. Alternative splicing results in multiple transcript variants encoding different isoforms. [provided by RefSeq
Other Designations
OTTHUMP00000001368|OTTHUMP00000044160
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Interactome
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Pathway
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Disease
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Publication Reference
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Sequestosome 1/p62 facilitates HER2-induced mammary tumorigenesis through multiple signaling pathways.
Cai-McRae X, Zhong H, Karantza V.
Oncogene 2015 Jun; 34(23):2968.
Application：WB-Tr, Mouse, Immortalized mouse mammary epithelial cells.
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Sequestosome 1/p62 facilitates HER2-induced mammary tumorigenesis through multiple signaling pathways.
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