MAPK3/MAPK1 (pT-E-pY Motif) monoclonal antibody, clone G15-B
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More Files
- More Functions
Western Blot (Transfected lysate)
Western Blot analysis of PC12 cell lysate Lane 1: untreated, Lane 2: treated with EGF (100 ng/mL, 5min), Lane 3: treated with PMA (100 nM, 30min) and Lane 4: in serum free DMEM and treated with EGF (100 ng/mL, 5min with 10% FBS in DMEM) with MAPK3/MAPK1 (pT-E-pY Motif) monoclonal antibody, clone G15-B (Cat # MAB15896).
Immunocytochemistry
Immunocytochemical staining of HEK293 cells with MAPK3/MAPK1 (pT-E-pY Motif) monoclonal antibody, clone G15-B (Cat # MAB15896) at 1:100 dilution.
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Specification
Product Description
Rabbit monoclonal antibody raised against synthetic phosphopeptide of human MAPK3/MAPK1.
Immunogen
A synthetic peptide corresponding to the protein area including conserved pT-E-pY motif of activated MAPK3/MAPK1.
Host
Rabbit
Reactivity
Human
Form
Liquid
Purification
EVAC purification
Isotype
IgG
Recommend Usage
ELISA (1:20000-1:100000)
Immunocytochemistry
Immunoprecipitation
Western Blot (1:5000)
The optimal working dilution should be determined by the end user.Storage Buffer
In 20 mM Tris-HCl buffer, pH 8.0 (10 mg/mL BSA, 0.05% Sodium Azide).
Storage Instruction
Store at -20°C.
Aliquot to avoid repeated freezing and thawing.Note
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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Applications
Western Blot (Transfected lysate)
Western Blot analysis of PC12 cell lysate Lane 1: untreated, Lane 2: treated with EGF (100 ng/mL, 5min), Lane 3: treated with PMA (100 nM, 30min) and Lane 4: in serum free DMEM and treated with EGF (100 ng/mL, 5min with 10% FBS in DMEM) with MAPK3/MAPK1 (pT-E-pY Motif) monoclonal antibody, clone G15-B (Cat # MAB15896).Immunocytochemistry
Immunocytochemical staining of HEK293 cells with MAPK3/MAPK1 (pT-E-pY Motif) monoclonal antibody, clone G15-B (Cat # MAB15896) at 1:100 dilution.Immunoprecipitation
Enzyme-linked Immunoabsorbent Assay
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Gene Info — MAPK1
Entrez GeneID
5594Protein Accession#
P27361;P28482Gene Name
MAPK1
Gene Alias
ERK, ERK2, ERT1, MAPK2, P42MAPK, PRKM1, PRKM2, p38, p40, p41, p41mapk
Gene Description
mitogen-activated protein kinase 1
Omim ID
176948Gene Ontology
HyperlinkGene Summary
The protein encoded by this gene is a member of the MAP kinase family. MAP kinases, also known as extracellular signal-regulated kinases (ERKs), act as an integration point for multiple biochemical signals, and are involved in a wide variety of cellular processes such as proliferation, differentiation, transcription regulation and development. The activation of this kinase requires its phosphorylation by upstream kinases. Upon activation, this kinase translocates to the nucleus of the stimulated cells, where it phosphorylates nuclear targets. Two alternatively spliced transcript variants encoding the same protein, but differing in the UTRs, have been reported for this gene. [provided by RefSeq
Other Designations
OTTHUMP00000174492|extracellular signal-regulated kinase 2|extracellular signal-regulated kinase-2|mitogen-activated protein kinase 2|protein tyrosine kinase ERK2
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Gene Info — MAPK3
Entrez GeneID
5595Protein Accession#
P27361;P28482Gene Name
MAPK3
Gene Alias
ERK1, HS44KDAP, HUMKER1A, MGC20180, P44ERK1, P44MAPK, PRKM3
Gene Description
mitogen-activated protein kinase 3
Omim ID
601795Gene Ontology
HyperlinkGene Summary
The protein encoded by this gene is a member of the MAP kinase family. MAP kinases, also known as extracellular signal-regulated kinases (ERKs), act in a signaling cascade that regulates various cellular processes such as proliferation, differentiation, and cell cycle progression in response to a variety of extracellular signals. This kinase is activated by upstream kinases, resulting in its translocation to the nucleus where it phosphorylates nuclear targets. Alternatively spliced transcript variants encoding different protein isoforms have been described. [provided by RefSeq
Other Designations
OTTHUMP00000174538|OTTHUMP00000174540|extracellular signal-regulated kinase 1|extracellular signal-related kinase 1
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Interactome
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Pathway
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Disease
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Publication Reference
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A Comparison of Phosphospecific Affinity Reagents Reveals the Utility of Recombinant Forkhead-associated Domains in Recognizing Phosphothreonine-Containing Peptides.
Venegas LA, Pershad K, Bankole O, Shah N, Kay BK.
New Biotechnology 2016 Sep; 33(5 Pt A):537.
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A Comparison of Phosphospecific Affinity Reagents Reveals the Utility of Recombinant Forkhead-associated Domains in Recognizing Phosphothreonine-Containing Peptides.
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