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MAPK3/MAPK1 (pT-E-pY Motif) monoclonal antibody, clone G15-B

  • Catalog # : MAB15896
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  • Specification
  • Product Description:
  • Rabbit monoclonal antibody raised against synthetic phosphopeptide of human MAPK3/MAPK1.
  • Immunogen:
  • A synthetic peptide corresponding to the protein area including conserved pT-E-pY motif of activated MAPK3/MAPK1.
  • Host:
  • Rabbit
  • Reactivity:
  • Human
  • Form:
  • Liquid
  • Purification:
  • EVAC purification
  • Isotype:
  • IgG
  • Recommend Usage:
  • ELISA (1:20000-1:100000)
    Immunocytochemistry
    Immunoprecipitation
    Western Blot (1:5000)
    The optimal working dilution should be determined by the end user.
  • Storage Buffer:
  • In 20 mM Tris-HCl buffer, pH 8.0 (10 mg/mL BSA, 0.05% Sodium Azide).
  • Storage Instruction:
  • Store at -20°C.
    Aliquot to avoid repeated freezing and thawing.
  • Note:
  • This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
  • Publication Reference
  • Applications
  • Western Blot (Transfected lysate)
  • Western Blot (Transfected lysate)
  • Western Blot analysis of PC12 cell lysate Lane 1: untreated, Lane 2: treated with EGF (100 ng/mL, 5min), Lane 3: treated with PMA (100 nM, 30min) and Lane 4: in serum free DMEM and treated with EGF (100 ng/mL, 5min with 10% FBS in DMEM) with MAPK3/MAPK1 (pT-E-pY Motif) monoclonal antibody, clone G15-B (Cat # MAB15896).
  • Immunocytochemistry
  • Immunocytochemistry
  • Immunocytochemical staining of HEK293 cells with MAPK3/MAPK1 (pT-E-pY Motif) monoclonal antibody, clone G15-B (Cat # MAB15896) at 1:100 dilution.
  • Immunoprecipitation
  • ELISA
  • Application Image
  • Western Blot (Transfected lysate)
  • Western Blot (Transfected lysate)
  • enlarge
  • Immunoprecipitation
  • ELISA
  • Gene Information
  • Entrez GeneID:
  • 5595
  • Protein Accession#:
  • P27361;P28482
  • Gene Name:
  • MAPK3
  • Gene Alias:
  • ERK1,HS44KDAP,HUMKER1A,MGC20180,P44ERK1,P44MAPK,PRKM3
  • Gene Description:
  • mitogen-activated protein kinase 3
  • Gene Summary:
  • The protein encoded by this gene is a member of the MAP kinase family. MAP kinases, also known as extracellular signal-regulated kinases (ERKs), act in a signaling cascade that regulates various cellular processes such as proliferation, differentiation, and cell cycle progression in response to a variety of extracellular signals. This kinase is activated by upstream kinases, resulting in its translocation to the nucleus where it phosphorylates nuclear targets. Alternatively spliced transcript variants encoding different protein isoforms have been described. [provided by RefSeq
  • Other Designations:
  • OTTHUMP00000174538,OTTHUMP00000174540,extracellular signal-regulated kinase 1,extracellular signal-related kinase 1
  • Gene Information
  • Entrez GeneID:
  • 5594
  • Protein Accession#:
  • P27361;P28482
  • Gene Name:
  • MAPK1
  • Gene Alias:
  • ERK,ERK2,ERT1,MAPK2,P42MAPK,PRKM1,PRKM2,p38,p40,p41,p41mapk
  • Gene Description:
  • mitogen-activated protein kinase 1
  • Gene Summary:
  • The protein encoded by this gene is a member of the MAP kinase family. MAP kinases, also known as extracellular signal-regulated kinases (ERKs), act as an integration point for multiple biochemical signals, and are involved in a wide variety of cellular processes such as proliferation, differentiation, transcription regulation and development. The activation of this kinase requires its phosphorylation by upstream kinases. Upon activation, this kinase translocates to the nucleus of the stimulated cells, where it phosphorylates nuclear targets. Two alternatively spliced transcript variants encoding the same protein, but differing in the UTRs, have been reported for this gene. [provided by RefSeq
  • Other Designations:
  • OTTHUMP00000174492,extracellular signal-regulated kinase 2,extracellular signal-regulated kinase-2,mitogen-activated protein kinase 2,protein tyrosine kinase ERK2
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