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Histone H2B (phospho S14) monoclonal antibody

  • Catalog # : MAB15850
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  • Specification
  • Product Description:
  • Mouse monoclonal antibody raised against synthetic peptide of Histone H2B (phospho S14).
  • Immunogen:
  • A synthetic peptide (conjugated with KLH) corresponding to Histone H2B, phosphorylated at serine 14 .
  • Host:
  • Mouse
  • Reactivity:
  • Human
  • Form:
  • Liquid
  • Purification:
  • Protein A purification
  • Isotype:
  • IgG1
  • Recommend Usage:
  • ELISA (1:100)
    Western Blot (1:200)
    Dot Blot (1:2000)
    The optimal working dilution should be determined by the end user.
  • Storage Buffer:
  • In PBS (0.05% sodium azide, 0.05% proclin 300).
  • Storage Instruction:
  • Store at -20°C. For long term storage store at -80°C.
    Aliquot to avoid repeated freezing and thawing.
  • Note:
  • This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
  • Applications
  • Western Blot (Cell lysate)
  • Western Blot (Cell lysate)
  • Western Blot (Cell lysate) analysis of 15 ug histone extracts of HeLa cells.
  • ELISA
  • ELISA
  • ELISA is a quantitative method used to determine the titer of the antibody using a serial dilution of antibody against Histone H2B (phospho S14). The antigen used was a peptide containing the histone modification of interest. By plotting the absorbance against the antibody dilution, the titer of the antibody was estimated to be 1:2000.
  • Dot Blot
  • Dot Blot
  • Cross reactivity test using the Histone H2B (phospho S14) antibody.
    Dot Blot analysis was performed with peptides containing other histone phosphorylations and the unmodified H2BS14. One hundred to 0.2 pmol of the respective peptides were spotted on a membrane. The antibody was used at a dilution of 1:2000. The figure shows a high specificity of the antibody for the modification of interest.
  • Application Image
  • Western Blot (Cell lysate)
  • Western Blot (Cell lysate)
  • enlarge
  • Gene Information
  • Entrez GeneID:
  • 8347
  • Gene Name:
  • HIST1H2BC
  • Gene Alias:
  • H2B.1,H2B/l,H2BFL,MGC104246,dJ221C16.3
  • Gene Description:
  • histone cluster 1, H2bc
  • Gene Summary:
  • Histones are basic nuclear proteins that are responsible for the nucleosome structure of the chromosomal fiber in eukaryotes. Two molecules of each of the four core histones (H2A, H2B, H3, and H4) form an octamer, around which approximately 146 bp of DNA is wrapped in repeating units, called nucleosomes. The linker histone, H1, interacts with linker DNA between nucleosomes and functions in the compaction of chromatin into higher order structures. This gene is intronless and encodes a member of the histone H2B family. Transcripts from this gene lack polyA tails but instead contain a palindromic termination element. This gene is found in the large histone gene cluster on chromosome 6. [provided by RefSeq
  • Other Designations:
  • H2B histone family, member L,OTTHUMP00000016141,histone 1, H2bc
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