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BAP1 monoclonal antibody

  • Catalog # : MAB15847
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  • Specification
  • Product Description:
  • Mouse monoclonal antibody raised against recombinant BAP1.
  • Immunogen:
  • Recombinant protein corresponding to human BAP1.
  • Host:
  • Mouse
  • Reactivity:
  • Human, Mouse
  • Form:
  • Liquid
  • Purification:
  • Protein G purification
  • Isotype:
  • IgG1, kappa
  • Recommend Usage:
  • Western Blot (1:1000-2000)
    Immunoprecipitation (1:400)
    Immunofluorescence (1:1000)
    The optimal working dilution should be determined by the end user.
  • Storage Buffer:
  • In PBS (0.05% sodium azide).
  • Storage Instruction:
  • Store at -20°C. For long term storage store at -80°C.
    Aliquot to avoid repeated freezing and thawing.
  • Note:
  • This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
  • Applications
  • Western Blot
  • Western Blot
  • Western Blot analysis of (1) 30 ug whole cell extracts of NB4 cells, (2) 30 ug whole cell extracts of mouse M1 cells.
  • Immunofluorescence
  • Immunofluorescence
  • Immunofluorescent staining of Hela cell line with antibody followed by an anti-mouse antibody conjugated to Alexa594 (left). The middle panel shows staining of the nuclei with DAPI. A merge of the two stainings (right).
  • Immunoprecipitation
  • Immunoprecipitation
  • IP was performed on 1 mg RIPA cell lysate from human NB4 cells (lane 1-3) or mouse M1 cells (lane 4-6) or an IgG negative control (lane 2 and 5).
    The samples were analysed by Western blot analysis. The input sample (30 ug RIPA lysate) was used as a positive control (lane 1 and 4).
  • Application Image
  • Gene Information
  • Entrez GeneID:
  • 8314
  • Gene Name:
  • BAP1
  • Gene Alias:
  • DKFZp686N04275,FLJ35406,FLJ37180,HUCEP-13,KIAA0272,UCHL2,hucep-6
  • Gene Description:
  • BRCA1 associated protein-1 (ubiquitin carboxy-terminal hydrolase)
  • Gene Summary:
  • The protein encoded by this gene localizes to the nucleus and it interacts with the RING finger domain of the breast cancer 1, early onset protein (BRCA1). This gene is thought to be a tumor suppressor gene that functions in the BRCA1 growth control pathway. There are multiple polyadenylation sites found in this gene. [provided by RefSeq
  • Other Designations:
  • BRCA1 associated protein-1,cerebral protein-13,cerebral protein-6,ubiquitin carboxy-terminal hydrolase
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