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EP300 monoclonal antibody

  • Catalog # : MAB15840
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  • Specification
  • Product Description:
  • Mouse monoclonal antibody raised against EP300.
  • Immunogen:
  • Human p300 by DNA immunization in which the C-terminus of the protein was cloned and expressed.
  • Host:
  • Mouse
  • Reactivity:
  • Human
  • Form:
  • Liquid
  • Purification:
  • Protein A purification
  • Isotype:
  • IgG3
  • Recommend Usage:
  • ChIP (5 ug/CHIP)
    The optimal working dilution should be determined by the end user.
  • Storage Buffer:
  • In PBS (0.05% sodium azide, 0.05% proclin 300).
  • Storage Instruction:
  • Store at -20°C. For long term storage store at -80°C.
    Aliquot to avoid repeated freezing and thawing.
  • Note:
  • This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
  • Applications
  • ChIP
  • ChIP
  • ChIP was performed using HeLa cells. A titration of the antibody consisting of 1, 2, 5 and 10 ug per ChIP experiment was analysed. IgG (2 ug/IP) was used as negative IP control. Quantitative PCR was performed with primers for two genomic regions near the ANKRD32 and IRS2 genes, used as positive controls, and for the coding region of the inactive MYOD1 gene and an intergeic region on chromosome 11, used as negative controls. The figure shows the recovery, expressed as a % of input (the relative amount of immunoprecipitated DNA compared to input DNA after qPCR analysis).
  • ChIP-Seq
  • ChIP-Seq
  • ChIP was performed with 5 ug of antibody. The figure shows the peak distribution along the complete sequence and a 3 mb region of chromosome 5 and in two regions surrounding the IRS2 and ANKRD32 positive control genes. The position of the amplicon used for ChIP-qPCR is indicated by an arrow.
  • Application Image
  • Gene Information
  • Entrez GeneID:
  • 2033
  • Gene Name:
  • EP300
  • Gene Alias:
  • KAT3B,p300
  • Gene Description:
  • E1A binding protein p300
  • Gene Summary:
  • This gene encodes the adenovirus E1A-associated cellular p300 transcriptional co-activator protein. It functions as histone acetyltransferase that regulates transcription via chromatin remodeling and is important in the processes of cell proliferation and differentiation. It mediates cAMP-gene regulation by binding specifically to phosphorylated CREB protein. This gene has also been identified as a co-activator of HIF1A (hypoxia-inducible factor 1 alpha), and thus plays a role in the stimulation of hypoxia-induced genes such as VEGF. Defects in this gene are a cause of Rubinstein-Taybi syndrome and may also play a role in epithelial cancer. [provided by RefSeq
  • Other Designations:
  • E1A-associated protein p300,E1A-binding protein, 300kD,OTTHUMP00000028668,histone acetyltransferase p300
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