EP300 monoclonal antibody
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Specifications
Product Description
Mouse monoclonal antibody raised against EP300.
Immunogen
Human p300 by DNA immunization in which the C-terminus of the protein was cloned and expressed.
Host
Mouse
Reactivity
Human
Form
Liquid
Purification
Protein A purification
Isotype
IgG3
Recommend Usage
ChIP (5 ug/CHIP)
The optimal working dilution should be determined by the end user.Storage Buffer
In PBS (0.05% sodium azide, 0.05% proclin 300).
Storage Instruction
Store at -20°C. For long term storage store at -80°C.
Aliquot to avoid repeated freezing and thawing.Note
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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Applications
ChIP
ChIP was performed using HeLa cells. A titration of the antibody consisting of 1, 2, 5 and 10 ug per ChIP experiment was analysed. IgG (2 ug/IP) was used as negative IP control. Quantitative PCR was performed with primers for two genomic regions near the ANKRD32 and IRS2 genes, used as positive controls, and for the coding region of the inactive MYOD1 gene and an intergeic region on chromosome 11, used as negative controls. The figure shows the recovery, expressed as a % of input (the relative amount of immunoprecipitated DNA compared to input DNA after qPCR analysis).ChIP-Seq
ChIP was performed with 5 ug of antibody. The figure shows the peak distribution along the complete sequence and a 3 mb region of chromosome 5 and in two regions surrounding the IRS2 and ANKRD32 positive control genes. The position of the amplicon used for ChIP-qPCR is indicated by an arrow. -
Gene Info — EP300
Entrez GeneID
2033Protein Accession#
Q09472Gene Name
EP300
Gene Alias
KAT3B, p300
Gene Description
E1A binding protein p300
Gene Ontology
HyperlinkGene Summary
This gene encodes the adenovirus E1A-associated cellular p300 transcriptional co-activator protein. It functions as histone acetyltransferase that regulates transcription via chromatin remodeling and is important in the processes of cell proliferation and differentiation. It mediates cAMP-gene regulation by binding specifically to phosphorylated CREB protein. This gene has also been identified as a co-activator of HIF1A (hypoxia-inducible factor 1 alpha), and thus plays a role in the stimulation of hypoxia-induced genes such as VEGF. Defects in this gene are a cause of Rubinstein-Taybi syndrome and may also play a role in epithelial cancer. [provided by RefSeq
Other Designations
E1A-associated protein p300|E1A-binding protein, 300kD|OTTHUMP00000028668|histone acetyltransferase p300
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Interactomes
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Pathways
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Diseases
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