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Last updated: 2017/10/15

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NRF1 monoclonal antibody

  • Catalog # : MAB15839
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  • Specification
  • Product Description:
  • Mouse monoclonal antibody raised against recombiant NRF1.
  • Immunogen:
  • Recombinant protein corresponding to human NRF1.
  • Host:
  • Mouse
  • Reactivity:
  • Human
  • Form:
  • Liquid
  • Purification:
  • Protein A purification
  • Isotype:
  • IgG2a
  • Recommend Usage:
  • Western Blot (1:1000)
    ChIP (2 ug/CHIP)
    The optimal working dilution should be determined by the end user.
  • Storage Buffer:
  • In PBS (0.05% sodium azide).
  • Storage Instruction:
  • Store at -20°C. For long term storage store at -80°C.
    Aliquot to avoid repeated freezing and thawing.
  • Note:
  • This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
  • Applications
  • ChIP
  • ChIP
  • ChIP was performed using K562 cells. A titration of the antibody consisting of 1, 2, 5 and 10 ug per ChIP experiment was analysed. IgG (2 ug/IP) was used as negative IP control. Quantitative PCR was performed with primers for the BRCA1 and AURKA promoters, used as positive controls, and for the coding region of the inactive MYOD1 gene and the Sat2 satellite repeat, used as negative controls. Yhe figure shows the recovery, expressed as a % of input (the relative amount of immunoprecipitated DNA compared to input DNA after qPCR analysis).
  • ChIP-Seq
  • ChIP-Seq
  • ChIP was performed with 2 ug of antibody. The figure shows the peak distribution along the complete sequence and a 700 kb region of chromosome 1 and in two regions surrounding the BRCA1 and AURKA positive control genes.
  • Western Blot (Cell lysate)
  • Western Blot (Cell lysate)
  • Western Blot (Cell lysate) analysis of whole cell extracts of K562 cells.
  • Application Image
  • Western Blot (Cell lysate)
  • Western Blot (Cell lysate)
  • enlarge
  • Gene Information
  • Entrez GeneID:
  • 4899
  • Gene Name:
  • NRF1
  • Gene Alias:
  • ALPHA-PAL
  • Gene Description:
  • nuclear respiratory factor 1
  • Gene Summary:
  • This gene encodes a protein that homodimerizes and functions as a transcription factor which activates the expression of some key metabolic genes regulating cellular growth and nuclear genes required for respiration, heme biosynthesis, and mitochondrial DNA transcription and replication. The protein has also been associated with the regulation of neurite outgrowth. Alternate transcriptional splice variants, which encode the same protein, have been characterized. Additional variants encoding different protein isoforms have been described but they have not been fully characterized. Confusion has occurred in bibliographic databases due to the shared symbol of NRF1 for this gene and for "nuclear factor (erythroid-derived 2)-like 1" which has an official symbol of NFE2L1. [provided by RefSeq
  • Other Designations:
  • OTTHUMP00000184912,alpha palindromic-binding protein
  • Gene Pathway
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