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Last updated: 2017/10/15

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His tag monoclonal antibody

  • Catalog # : MAB15838
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  • Specification
  • Product Description:
  • Mouse monoclonal antibody raised against synthetic peptide of His tag.
  • Immunogen:
  • A synthetic peptide (conjugated with KLH) corresponding to 6xHis tag.
  • Sequence:
  • HHHHHH
  • Host:
  • Mouse
  • Specificity:
  • Specific for His-tagged fusion proteins expressed in bacteria, insect, mammalian cells.
  • Form:
  • Liquid
  • Purification:
  • Protein A purification
  • Isotype:
  • IgG2b
  • Recommend Usage:
  • Western Blot (1:1000-3000)
    ChIP/IP (1:150-200)
    Immunostaining (1:500-1000)
    The optimal working dilution should be determined by the end user.
  • Storage Buffer:
  • In PBS, pH 7.2.
  • Storage Instruction:
  • Store at -20°C. For long term storage store at -80°C.
    Aliquot to avoid repeated freezing and thawing.
  • Applications
  • ChIP
  • Western Blot (Transfected lysate)
  • Western Blot (Transfected lysate)
  • Western Blot (Cell lysate) analysis of (1) HEK293 cells transfected with a HA-tagged protein, and (2) Negative control (vector only transfected HEK293 cells). A single band is clearly visible in lane 2, but absent in lane 1 showing the high specificity of the HA antibody.
  • Western Blot
  • Western Blot
  • Western Blot analysis of bacterial extracts containing a His fusion protein.
  • Immunostaining
  • Application Image
  • ChIP
  • Western Blot (Transfected lysate)
  • Western Blot (Transfected lysate)
  • enlarge
  • Immunostaining
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