Histone H4 (K20me3) monoclonal antibody
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More Files
- More Functions
ChIP
ChIP assays were performed using human HeLa cells. 3 and 12 ul of antibody and 1 ug IgG (negative IP control) were analysed. QPCR was performed with primers for the GAPDH promoter and for the inactive gene TSH2B. The figure shows the recovery, expressed as a % of input (the relative amount of immunoprecipitated DNA compared to input DNA after qPCR analysis).
Western Blot (Cell lysate)
Western Blot (Cell lysate) analysis of 15 ug histone extracts of HeLa cells.
Immunofluorescence
Immunofluorescent staining of HEK293T cell line with antibody were labeled with goat-anti-mouse antibody conjugated to Alexa488 and goat-anti-human antibody conjugated to Alexa633. Cells were mounted with Vectashield containing DAPI.
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Specification
Product Description
Mouse monoclonal antibody raised against synthetic peptide of Histone H4 (K20me3).
Immunogen
A synthetic peptide (conjugated with KLH) corresponding to Histone H4, trimethylated at lysine 20.
Host
Mouse
Reactivity
Human, Zebra fish
Form
Liquid
Purification
Concentrated supernatant
Isotype
IgG1
Recommend Usage
Western Blot (1:50)
ChIP (3 ul/CHIP)
Immunofluorescence (1:200)
The optimal working dilution should be determined by the end user.Storage Buffer
In mouse hybridoma cell culture concentrated supernatant (0.05% sodium azide).
Storage Instruction
Store at -20°C. For long term storage store at -80°C.
Aliquot to avoid repeated freezing and thawing.Note
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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Applications
ChIP
ChIP assays were performed using human HeLa cells. 3 and 12 ul of antibody and 1 ug IgG (negative IP control) were analysed. QPCR was performed with primers for the GAPDH promoter and for the inactive gene TSH2B. The figure shows the recovery, expressed as a % of input (the relative amount of immunoprecipitated DNA compared to input DNA after qPCR analysis).Western Blot (Cell lysate)
Western Blot (Cell lysate) analysis of 15 ug histone extracts of HeLa cells.Immunofluorescence
Immunofluorescent staining of HEK293T cell line with antibody were labeled with goat-anti-mouse antibody conjugated to Alexa488 and goat-anti-human antibody conjugated to Alexa633. Cells were mounted with Vectashield containing DAPI. -
Gene Info — HIST1H4A
Entrez GeneID
8359Protein Accession#
P62805Gene Name
HIST1H4A
Gene Alias
H4/a, H4FA
Gene Description
histone cluster 1, H4a
Omim ID
602822Gene Ontology
HyperlinkGene Summary
Histones are basic nuclear proteins that are responsible for the nucleosome structure of the chromosomal fiber in eukaryotes. Two molecules of each of the four core histones (H2A, H2B, H3, and H4) form an octamer, around which approximately 146 bp of DNA is wrapped in repeating units, called nucleosomes. The linker histone, H1, interacts with linker DNA between nucleosomes and functions in the compaction of chromatin into higher order structures. This gene is intronless and encodes a member of the histone H4 family. Transcripts from this gene lack polyA tails but instead contain a palindromic termination element. This gene is found in the large histone gene cluster on chromosome 6. [provided by RefSeq
Other Designations
H4 histone family, member A|histone 1, H4a
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Interactome
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Pathway
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Publication Reference
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Role of annexin gene and its regulation during zebrafish caudal fin regeneration.
Sandeep Saxena, Sruthi Purushothaman, Vuppalapaty Meghah, Bhawna Bhatti, Akhila Poruri, Mula G Meena Lakshmi, Nukala Sarath Babu, Ch Lakshmi Narasimha Murthy, Komal K Mandal, Arvind Kumar, Mohammed M Idris.
Wound Repair and Regeneration 2016 May; 24(3):551.
Application：ChIP, Zebrafish, Caudal fin.
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Role of annexin gene and its regulation during zebrafish caudal fin regeneration.
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