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Histone H4 (K20me2) monoclonal antibody

  • Catalog # : MAB15830
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  • Specification
  • Product Description:
  • Mouse monoclonal antibody raised against synthetic peptide of Histone H4 (K20me2).
  • Immunogen:
  • A synthetic peptide (conjugated with KLH) corresponding to human histone H4, dimethylated at lysine 20.
  • Host:
  • Mouse
  • Reactivity:
  • Human
  • Form:
  • Liquid
  • Purification:
  • Protein A purification
  • Isotype:
  • IgG1
  • Recommend Usage:
  • Western Blot (1:1000)
    ChIP (1-5 ug/CHIP)
    Dot Blot (1:20000)
    Immunofluorescence (1:1000)
    The optimal working dilution should be determined by the end user.
  • Storage Buffer:
  • In PBS (0.05% sodium azide).
  • Storage Instruction:
  • Store at -20°C. For long term storage store at -80°C.
    Aliquot to avoid repeated freezing and thawing.
  • Note:
  • This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
  • Applications
  • ChIP
  • ChIP
  • ChIP assays were performed using HeLa cells. 1 and 5 ug of antibody and 1 ug IgG (negative IP control) were used per ChIP experiment. QPCR was performed with primers for the promoters of the GAPDH and EIF4A2 genes, used as negative controls, and for the Sat2 and SatA satellite repeats, used as positive controls. The figure shows the recovery, expressed as a % of input (the relative amount of immunoprecipitated DNA compared to input DNA after qPCR analysis).
  • Western Blot (Cell lysate)
  • Western Blot (Cell lysate)
  • Western Blot (Cell lysate) analysis of 15 ug histone extracts of HeLa cells.
  • Immunofluorescence
  • Immunofluorescence
  • Immunofluorescent staining of Hela cell line with antibody followed by an anti-mouse antibody conjugated to Alexa594 (middle). The left panel shows staining of the nuclei with DAPI. A merge of the two stainings (right).
  • Dot Blot
  • Dot Blot
  • Cross reactivity tests using the Histone H4 (K20me2) antibody.
    Dot Blot analysis was performed with peptides containing other histone modifications and the unmodified H4K20. One hundred to 0.2 pmol of the respective peptides were spotted on a membrane. The antibody was used at a dilution of 1:20000. The figure shows a high specificity of the antibody for the modification of interest.
  • Application Image
  • Western Blot (Cell lysate)
  • Western Blot (Cell lysate)
  • enlarge
  • Gene Information
  • Entrez GeneID:
  • 8359
  • Gene Name:
  • HIST1H4A
  • Gene Alias:
  • H4/a,H4FA
  • Gene Description:
  • histone cluster 1, H4a
  • Gene Summary:
  • Histones are basic nuclear proteins that are responsible for the nucleosome structure of the chromosomal fiber in eukaryotes. Two molecules of each of the four core histones (H2A, H2B, H3, and H4) form an octamer, around which approximately 146 bp of DNA is wrapped in repeating units, called nucleosomes. The linker histone, H1, interacts with linker DNA between nucleosomes and functions in the compaction of chromatin into higher order structures. This gene is intronless and encodes a member of the histone H4 family. Transcripts from this gene lack polyA tails but instead contain a palindromic termination element. This gene is found in the large histone gene cluster on chromosome 6. [provided by RefSeq
  • Other Designations:
  • H4 histone family, member A,histone 1, H4a
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