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Last updated: 2018/1/14

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CRISPR-Cas9 monoclonal antibody

  • Catalog # : MAB15818
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  • Specification
  • Product Description:
  • Mouse monoclonal antibody raised against recombinant CRISPR-Cas9.
  • Immunogen:
  • Recombinant protein corresponding to N-terminus of the Cas9 nuclease (CRISPR-associated protein 9).
  • Host:
  • Mouse
  • Reactivity:
  • Streptococcus pyogenes
  • Form:
  • Liquid
  • Purification:
  • Protein A purification
  • Isotype:
  • IgG1, kappa
  • Recommend Usage:
  • Western Blot (1:1000-1:6000)
    Immunoprecipitation (1:200)
    Immunofluorescence (1:100-500)
    The optimal working dilution should be determined by the end user.
  • Storage Buffer:
  • In PBS (0.02% sodium azide).
  • Storage Instruction:
  • Store at -20°C. For long term storage store at -80°C.
    Aliquot to avoid repeated freezing and thawing.
  • Note:
  • This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
  • Publication Reference
  • Applications
  • Western Blot (Cell lysate)
  • Western Blot (Cell lysate)
  • Western Blot (Cell lysate) analysis of (1) HeLa cells transfected with a flag-tagged Cas9, (2) 10 ng of recombinant Cas9 protein, and (3) 1 ng of recombinant Cas9 protein.
  • Immunofluorescence
  • Immunofluorescence
  • Immunofluorescent staining of Hela cell line with antibody followed by incubation with an anti mouse secondary antibody (left). Nuclei were counter-stained with DAPI (right).
  • Immunoprecipitation
  • Immunoprecipitation
  • IP was performed on whole cell extracts (100 ug) from HEK293 cells transfected with a Flag-tagged Cas9.
    The immunoprecipitated proteins were subsequently analysed by Western Blot with the antibody. Lane 3 and 4 show the result of the IP; a negative IP control (IP on untransfected cells) and the input (15 ug) are shown in lane 2 and 1, respectively.
  • Application Image
  • Western Blot (Cell lysate)
  • Western Blot (Cell lysate)
  • enlarge
  • Gene Information
  • Gene Name:
  • SPy_1046
  • Gene Alias:
  • -
  • Gene Description:
  • hypothetical protein
  • Other Designations:
  • -
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