CEP350 monoclonal antibody, clone CL3423
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More Files
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Immunohistochemical staining (Formalin-fixed paraffin-embedded sections) of human colon with CEP350 monoclonal antibody, clone CL3423 (Cat # MAB15801) shows centrosome-like positivity in lymphoid cells in lamina propria.
Immunofluorescence
Immunofluorescent staining of A431 cells with CEP350 monoclonal antibody, clone CL3423 (Cat # MAB15801) (Green) shows specific the centrosome. Microtubule and nuclear probes are visualized in red and blue, respectively (where available).
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Specification
Product Description
Mouse monoclonal antibody raised against partial recombinant human CEP350.
Immunogen
Recombinant protein corresponding to human CEP350.
Epitope
This antibody binds to an epitope located within the peptide sequence LPDFVKQLRTRT as determined by overlapping synthetic peptides.
Sequence
QVVQSQREVTEVLQEATCKIAAQQSETARLTTDAARQICEMAELTRTHISDAVVASGAPLAILYDHQRQHLPDFVKQLRTRTETDRKSPSVSLSQ
Host
Mouse
Reactivity
Human
Form
Liquid
Purification
Protein A purification
Isotype
IgG1
Recommend Usage
Immunofluorescence (1-4 ug/mL)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) (1:50-1:200)
The optimal working dilution should be determined by the end user.Storage Buffer
In PBS, pH 7.2 (40% glycerol, 0.02% sodium azide).
Storage Instruction
Store at 4°C. For long term storage store at -20°C.
Aliquot to avoid repeated freezing and thawing.Note
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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Applications
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Immunohistochemical staining (Formalin-fixed paraffin-embedded sections) of human colon with CEP350 monoclonal antibody, clone CL3423 (Cat # MAB15801) shows centrosome-like positivity in lymphoid cells in lamina propria.Immunofluorescence
Immunofluorescent staining of A431 cells with CEP350 monoclonal antibody, clone CL3423 (Cat # MAB15801) (Green) shows specific the centrosome. Microtubule and nuclear probes are visualized in red and blue, respectively (where available). -
Gene Info — CEP350
Entrez GeneID
9857Protein Accession#
Q5VT06Gene Name
CEP350
Gene Alias
CAP350, FLJ38282, FLJ44058, GM133, KIAA0480
Gene Description
centrosomal protein 350kDa
Gene Ontology
HyperlinkGene Summary
The product of this gene is a large protein with a CAP-Gly domain typically found in cytoskeleton-associated proteins. The encoded protein primarily localizes to the centrosome, a non-membraneous organelle that functions as the major microtubule-organizing center in animal cells. The encoded protein directly interacts with another large centrosomal protein and is required to anchor microtubules at the centrosome. It is also implicated in the regulation of a class of nuclear hormone receptors in the nucleus. Several alternatively spliced transcript variants have been found, but their full-length nature has not been determined. [provided by RefSeq
Other Designations
OTTHUMP00000060646|centrosome associated protein 350|centrosome-associated protein 350
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Interactome
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Disease
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