PGM1 monoclonal antibody, clone CL3299
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Specification
Product Description
Mouse monoclonal antibody raised against partial recombinant human PGM1.
Immunogen
Recombinant protein corresponding to human PGM1.
Epitope
This antibody binds to an epitope located within the peptide sequence HGRNFFTRYD as determined by overlapping synthetic peptides.
Sequence
SILATRKQSVEDILKDHWQKHGRNFFTRYDYEEVEAEGANKMMKDLEALMFDRSFVGKQFSANDKVYTVEKADNFEYSD
Host
Mouse
Reactivity
Human
Form
Liquid
Purification
Protein A purification
Isotype
IgG1
Recommend Usage
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) (1:2500-1:5000)
Western Blot (1:500-1:1000)
The optimal working dilution should be determined by the end user.Storage Buffer
In PBS, pH 7.2 (40% glycerol, 0.02% sodium azide).
Storage Instruction
Store at 4°C. For long term storage store at -20°C.
Aliquot to avoid repeated freezing and thawing.Note
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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Applications
Western Blot (Cell lysate)
Western Blot analysis of RT-4 cell lysate with PGM1 monoclonal antibody, clone CL3299 (Cat # MAB15798).Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Immunohistochemical staining (Formalin-fixed paraffin-embedded sections) of human skeletal muscle with PGM1 monoclonal antibody, clone CL3299 (Cat # MAB15798) shows moderate cytoplasmic immunoreactivity in muscle fibers. -
Gene Info — PGM1
Entrez GeneID
5236Protein Accession#
P36871Gene Name
PGM1
Gene Alias
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Gene Description
phosphoglucomutase 1
Omim ID
171900Gene Ontology
HyperlinkGene Summary
Phosphoglucomutases (PGM; EC 5.4.2.2) catalyze the transfer of phosphate between the 1 and 6 positions of glucose. Isozymes of PGM are monomeric, with molecular masses of about 60 kD, and are encoded by several genes, including PGM1. In most cell types, PGM1 isozymes predominate, representing about 90% of total PGM activity. One exception is red cells, where PGM2 (MIM 172000) is a major isozyme (Putt et al., 1993 [PubMed 8257433]).[supplied by OMIM
Other Designations
OTTHUMP00000010519|OTTHUMP00000046842
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Interactome
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