UCHL1 monoclonal antibody, clone CL3210
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More Files
- More Functions
Western Blot (Tissue lysate)
Western Blot analysis of human cerebral cortex tissue lysate with UCHL1 monoclonal antibody, clone CL3210 (Cat # MAB15796).
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Immunohistochemical staining (Formalin-fixed paraffin-embedded sections) of human cerebellum with UCHL1 monoclonal antibody, clone CL3210 (Cat # MAB15796) shows moderate positivity in Purkinje cells.
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Specification
Product Description
Mouse monoclonal antibody raised against partial recombinant human UCHL1.
Immunogen
Recombinant protein corresponding to human UCHL1.
Epitope
This antibody binds to an epitope located within the peptide sequence AVANNQDKLG as determined by overlapping synthetic peptides.
Sequence
QEVSPKVYFMKQTIGNSCGTIGLIHAVANNQDKLGFEDGSVLKQFLSETEKMSPEDRAKCFEKNEAIQAAHDAVAQEGQCRVDDKVNFHFILFNNVDGHLYELDGRMPFPVNHGASSEDTLLKDAAKVCREFTEREQGEVRFSAVAL
Host
Mouse
Reactivity
Human, Mouse, Rat
Form
Liquid
Purification
Protein A purification
Isotype
IgG1
Recommend Usage
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) (1:5000-1:10000)
Western Blot (1:500-1:1000)
The optimal working dilution should be determined by the end user.Storage Buffer
In PBS, pH 7.2 (40% glycerol, 0.02% sodium azide).
Storage Instruction
Store at 4°C. For long term storage store at -20°C.
Aliquot to avoid repeated freezing and thawing.Note
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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Applications
Western Blot (Tissue lysate)
Western Blot analysis of human cerebral cortex tissue lysate with UCHL1 monoclonal antibody, clone CL3210 (Cat # MAB15796).Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Immunohistochemical staining (Formalin-fixed paraffin-embedded sections) of human cerebellum with UCHL1 monoclonal antibody, clone CL3210 (Cat # MAB15796) shows moderate positivity in Purkinje cells. -
Gene Info — UCHL1
Entrez GeneID
7345Protein Accession#
P09936Gene Name
UCHL1
Gene Alias
PARK5, PGP9.5, Uch-L1
Gene Description
ubiquitin carboxyl-terminal esterase L1 (ubiquitin thiolesterase)
Gene Ontology
HyperlinkGene Summary
The protein encoded by this gene belongs to the peptidase C12 family. This enzyme is a thiol protease that hydrolyzes a peptide bond at the C-terminal glycine of ubiquitin. This gene is specifically expressed in the neurons and in cells of the diffuse neuroendocrine system. Mutations in this gene may be associated with Parkinson disease
Other Designations
ubiquitin C-terminal esterase L1|ubiquitin carboxyl-terminal esterase L1|ubiquitin thiolesterase L1
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Interactome
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Disease
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