NOP56 monoclonal antibody, clone CL2603
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More Files
- More Functions
Western Blot (Cell lysate)
Western Blot analysis of U-251 MG cell lysate with NOP56 monoclonal antibody, clone CL2603 (Cat # MAB15761).
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Immunohistochemical staining (Formalin-fixed paraffin-embedded sections) of human prostate cancer with NOP56 monoclonal antibody, clone CL2603 (Cat # MAB15761) shows strong nucleolar positivity in glandular cells.
Immunofluorescence
Immunofluorescent staining of MCF7 cells with NOP56 monoclonal antibody, clone CL2603 (Cat # MAB15761) (Green) shows specific nucleoli. Microtubule and nuclear probes are visualized in red and blue respectively (where available).
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Specification
Product Description
Mouse monoclonal antibody raised against partial recombinant human NOP56.
Immunogen
Recombinant protein corresponding to human NOP56.
Epitope
This antibody binds to an epitope located within the peptide sequence FSKEELMSSDLEETA as determined by overlapping synthetic peptides.
Sequence
EDPSISFSKPKKKKSFSKEELMSSDLEETAGSTS
Host
Mouse
Reactivity
Human
Form
Liquid
Purification
Protein A purification
Isotype
IgG1
Recommend Usage
Immunofluorescence (2-10 ug/mL)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) (1:500-1:1000)
Western Blot (1:500-1:1000)
The optimal working dilution should be determined by the end user.Storage Buffer
In PBS, pH 7.2 (40% glycerol, 0.02% sodium azide).
Storage Instruction
Store at 4°C. For long term storage store at -20°C.
Aliquot to avoid repeated freezing and thawing.Note
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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Applications
Western Blot (Cell lysate)
Western Blot analysis of U-251 MG cell lysate with NOP56 monoclonal antibody, clone CL2603 (Cat # MAB15761).Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Immunohistochemical staining (Formalin-fixed paraffin-embedded sections) of human prostate cancer with NOP56 monoclonal antibody, clone CL2603 (Cat # MAB15761) shows strong nucleolar positivity in glandular cells.Immunofluorescence
Immunofluorescent staining of MCF7 cells with NOP56 monoclonal antibody, clone CL2603 (Cat # MAB15761) (Green) shows specific nucleoli. Microtubule and nuclear probes are visualized in red and blue respectively (where available).
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