EZR monoclonal antibody, clone CL2384
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Specification
Product Description
Mouse monoclonal antibody raised against partial recombinant human EZR.
Immunogen
Recombinant protein corresponding to human EZR.
Epitope
This antibody binds to an epitope located within the peptide sequence LSEQIQRALQLEEER as determined by overlapping synthetic peptides.
Sequence
REEKHQKQLERQQLETEKKRRETVEREKEQMMREKEELMLRLQDYEEKTKKAERELSEQIQRALQLEEERKRAQEEAERLEADRMAALRAKEELERQAVDQIKSQEQLAAELAEYTAKIALLE
Host
Mouse
Reactivity
Human
Form
Liquid
Purification
Protein A purification
Isotype
IgG1
Recommend Usage
Immunofluorescence (1-4 ug/mL)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) (1:5000-1:10000)
Western Blot (1:500-1:1000)
The optimal working dilution should be determined by the end user.Storage Buffer
In PBS, pH 7.2 (40% glycerol, 0.02% sodium azide).
Storage Instruction
Store at 4°C. For long term storage store at -20°C.
Aliquot to avoid repeated freezing and thawing.Note
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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Applications
Western Blot (Cell lysate)
Western Blot analysis of U-251 MG cell lysate with EZR monoclonal antibody, clone CL2384 (Cat # MAB15743).Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Immunohistochemical staining (Formalin-fixed paraffin-embedded sections) of human prostate cancer with EZR monoclonal antibody, clone CL2384 (Cat # MAB15743) shows strong membranous and moderate cytoplasmic positivity in glandular cells.Immunofluorescence
Immunofluorescent staining of MCF7 cells with EZR monoclonal antibody, clone CL2384 (Cat # MAB15743) (Green) shows specific plasma membrane. Microtubule and nuclear probes are visualized in red and blue respectively (where available). -
Gene Info — EZR
Entrez GeneID
7430Protein Accession#
P15311Gene Name
EZR
Gene Alias
CVIL, CVL, DKFZp762H157, FLJ26216, MGC1584, VIL2
Gene Description
ezrin
Omim ID
123900Gene Ontology
HyperlinkGene Summary
The cytoplasmic peripheral membrane protein encoded by this gene functions as a protein-tyrosine kinase substrate in microvilli. As a member of the ERM protein family, this protein serves as an intermediate between the plasma membrane and the actin cytoskeleton. This protein plays a key role in cell surface structure adhesion, migration and organization, and it has been implicated in various human cancers. A pseudogene located on chromosome 3 has been identified for this gene. Alternatively spliced variants have also been described for this gene. [provided by RefSeq
Other Designations
OTTHUMP00000017506|OTTHUMP00000017507|cytovillin 2|villin 2 (ezrin)
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Interactome
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