TUFM monoclonal antibody, clone CL2245
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More Files
- More Functions
Western Blot (Cell lysate)
Western Blot analysis of U-251 MG cell lysate with TUFM monoclonal antibody, clone CL2245 (Cat # MAB15736).
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Immunohistochemical staining (Formalin-fixed paraffin-embedded sections) of human skin with TUFM monoclonal antibody, clone CL2245 (Cat # MAB15736) shows granular cytoplasmic immunoreactivity in epithelial cells.
Immunofluorescence
Immunofluorescent staining of MCF7 cells with TUFM monoclonal antibody, clone CL2245 (Cat # MAB15736) (Green) shows distinct mitochondrial. Microtubule and nuclear probes are visualized in red and blue respectively (where available).
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Specification
Product Description
Mouse monoclonal antibody raised against partial recombinant human TUFM.
Immunogen
Recombinant protein corresponding to human TUFM.
Epitope
This antibody binds to an epitope located within the peptide sequence PFLLPVEAVYSVPGR as determined by overlapping synthetic peptides.
Sequence
AVDTYIPVPARDLEKPFLLPVEAVYSVPGRGTVVTGTLERGILKKGDECELLGHSKNIRTVVTGIEMFHKSLERAEAGDNLGALVRGLKREDLRRGLVMVKPGSI
Host
Mouse
Reactivity
Human
Form
Liquid
Purification
Protein A purification
Isotype
IgG1
Recommend Usage
Immunofluorescence (1-4 ug/mL)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) (1:5000-1:10000)
Western Blot (1:500-1:1000)
The optimal working dilution should be determined by the end user.Storage Buffer
In PBS, pH 7.2 (40% glycerol, 0.02% sodium azide).
Storage Instruction
Store at 4°C. For long term storage store at -20°C.
Aliquot to avoid repeated freezing and thawing.Note
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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Applications
Western Blot (Cell lysate)
Western Blot analysis of U-251 MG cell lysate with TUFM monoclonal antibody, clone CL2245 (Cat # MAB15736).Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Immunohistochemical staining (Formalin-fixed paraffin-embedded sections) of human skin with TUFM monoclonal antibody, clone CL2245 (Cat # MAB15736) shows granular cytoplasmic immunoreactivity in epithelial cells.Immunofluorescence
Immunofluorescent staining of MCF7 cells with TUFM monoclonal antibody, clone CL2245 (Cat # MAB15736) (Green) shows distinct mitochondrial. Microtubule and nuclear probes are visualized in red and blue respectively (where available). -
Gene Info — TUFM
Entrez GeneID
7284Protein Accession#
P49411Gene Name
TUFM
Gene Alias
COXPD4, EF-TuMT, EFTU, P43
Gene Description
Tu translation elongation factor, mitochondrial
Gene Ontology
HyperlinkGene Summary
This gene encodes a protein which participates in protein translation in mitochondria. Mutations in this gene have been associated with combined oxidative phosphorylation deficiency resulting in lactic acidosis and fatal encephalopathy. A pseudogene has been identified on chromosome 17. [provided by RefSeq
Other Designations
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Interactome
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