MKI67IP monoclonal antibody, clone CL2240
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Specification
Product Description
Mouse monoclonal antibody raised against partial recombinant human MKI67IP.
Immunogen
Recombinant protein corresponding to human MKI67IP.
Epitope
This antibody binds to an epitope located within the peptide sequence KVSGTLDTPE as determined by overlapping synthetic peptides.
Sequence
IDYDFPSLILQKTESISKTNRQTSTKGQVLRKKKKKVSGTLDTPEKTVDSQGPTPVCTPTFLERRKSQVAELNDDDKDDEI
Host
Mouse
Reactivity
Human
Form
Liquid
Purification
Protein A purification
Isotype
IgG2a
Recommend Usage
Immunofluorescence (1-4 ug/mL)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) (1:200-1:500)
The optimal working dilution should be determined by the end user.Storage Buffer
In PBS, pH 7.2 (40% glycerol, 0.02% sodium azide).
Storage Instruction
Store at 4°C. For long term storage store at -20°C.
Aliquot to avoid repeated freezing and thawing.Note
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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Applications
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Immunohistochemical staining (Formalin-fixed paraffin-embedded sections) of human fallopian tube with MKI67IP monoclonal antibody, clone CL2240 (Cat # MAB15732) shows strong nucleolar immunoreactivity in glandular and connective tissue cells.Immunofluorescence
Immunofluorescent staining of MCF7 cells with MKI67IP monoclonal antibody, clone CL2240 (Cat # MAB15732) (Green) shows specific nucleoli. Microtubule and nuclear probes are visualized in red and blue respectively (where available). -
Gene Info — MKI67IP
Entrez GeneID
84365Protein Accession#
Q9BYG3Gene Name
MKI67IP
Gene Alias
NIFK, Nopp34
Gene Description
MKI67 (FHA domain) interacting nucleolar phosphoprotein
Gene Ontology
HyperlinkGene Summary
This gene encodes a protein that interacts with the forkhead-associated domain of the Ki-67 antigen. The encoded protein may bind RNA and may play a role in mitosis and cell cycle progression. Multiple pseudogenes exist on chromosomes 5, 10, 12, 15, and 19.[provided by RefSeq
Other Designations
nucleolar phosphoprotein Nopp34|nucleolar protein interacting with the FHA domain of pKi-67
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