PARP1 monoclonal antibody, clone CL2220
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More Files
- More Functions
Western Blot (Cell lysate)
Western Blot analysis of RT-4 cell lysate with PARP1 monoclonal antibody, clone CL2220 (Cat # MAB15730).
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Immunohistochemical staining (Formalin-fixed paraffin-embedded sections) of human fallopian tube with PARP1 monoclonal antibody, clone CL2220 (Cat # MAB15730) shows strong nuclear immunoreactivity.
Immunofluorescence
Immunofluorescent staining of MCF7 cells with PARP1 monoclonal antibody, clone CL2220 (Cat # MAB15730) (Green) shows clear cycle dependent nuclear. Microtubule and nuclear probes are visualized in red and blue respectively (where available).
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Specification
Product Description
Mouse monoclonal antibody raised against partial recombinant human PARP1.
Immunogen
Recombinant protein corresponding to human PARP1.
Epitope
This antibody binds to an epitope located within the peptide sequence SKLPKPVQDL as determined by overlapping synthetic peptides.
Sequence
KGGKVFSATLGLVDIVKGTNSYYKLQLLEDDKENRYWIFRSWGRVGTVIGSNKLEQMPSKEDAIEHFMKLYEEKTGNAWHSKNFTKYPKKFYPLEIDYGQDEEAVKKLTVNPGTKSKLPKPVQDLIKMIFDVESMKKAM
Host
Mouse
Reactivity
Human
Form
Liquid
Purification
Protein A purification
Isotype
IgG1
Recommend Usage
Immunofluorescence (1-4 ug/mL)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) (1:1000-1:2500)
Western Blot (1:500-1:1000)
The optimal working dilution should be determined by the end user.Storage Buffer
In PBS, pH 7.2 (40% glycerol, 0.02% sodium azide).
Storage Instruction
Store at 4°C. For long term storage store at -20°C.
Aliquot to avoid repeated freezing and thawing.Note
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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Applications
Western Blot (Cell lysate)
Western Blot analysis of RT-4 cell lysate with PARP1 monoclonal antibody, clone CL2220 (Cat # MAB15730).Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Immunohistochemical staining (Formalin-fixed paraffin-embedded sections) of human fallopian tube with PARP1 monoclonal antibody, clone CL2220 (Cat # MAB15730) shows strong nuclear immunoreactivity.Immunofluorescence
Immunofluorescent staining of MCF7 cells with PARP1 monoclonal antibody, clone CL2220 (Cat # MAB15730) (Green) shows clear cycle dependent nuclear. Microtubule and nuclear probes are visualized in red and blue respectively (where available). -
Gene Info — PARP1
Entrez GeneID
142Protein Accession#
P09874Gene Name
PARP1
Gene Alias
ADPRT, ADPRT1, PARP, PARP-1, PPOL, pADPRT-1
Gene Description
poly (ADP-ribose) polymerase 1
Omim ID
173870Gene Ontology
HyperlinkGene Summary
This gene encodes a chromatin-associated enzyme, poly(ADP-ribosyl)transferase, which modifies various nuclear proteins by poly(ADP-ribosyl)ation. The modification is dependent on DNA and is involved in the regulation of various important cellular processes such as differentiation, proliferation, and tumor transformation and also in the regulation of the molecular events involved in the recovery of cell from DNA damage. In addition, this enzyme may be the site of mutation in Fanconi anemia, and may participate in the pathophysiology of type I diabetes. [provided by RefSeq
Other Designations
ADP-ribosyltransferase (NAD+; poly (ADP-ribose) polymerase)|ADP-ribosyltransferase NAD(+)|OTTHUMP00000035663|poly (ADP-ribose) polymerase family, member 1|poly(ADP-ribose) polymerase|poly(ADP-ribose) synthetase|poly(ADP-ribosyl)transferase
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Interactome
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Pathway
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Disease
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