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Last updated: 2017/10/15

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PPIB monoclonal antibody, clone CL3901

  • Catalog # : MAB15696
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  • Specification
  • Product Description:
  • Mouse monoclonal antibody raised against synthetic peptide of human PPIB.
  • Immunogen:
  • A synthetic peptide corresponding to human PPIB.
  • Sequence:
  • TKTDSRDKPLKDVIIADCGKIEVEKPFAIAKE
  • Host:
  • Mouse
  • Reactivity:
  • Human, Mouse, Rat
  • Form:
  • Liquid
  • Purification:
  • Protein A purification
  • Isotype:
  • IgG2a
  • Recommend Usage:
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) (1:5000-1:10000)
    Western Blot (1:500-1:1000)
    The optimal working dilution should be determined by the end user.
  • Storage Buffer:
  • In PBS, pH 7.2 (40% glycerol, 0.02% sodium azide).
  • Storage Instruction:
  • Store at 4°C. For long term storage store at -20°C.
    Aliquot to avoid repeated freezing and thawing.
  • Note:
  • This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
  • Applications
  • Western Blot
  • Western Blot
  • Western Blot (Cell lysate) analysis of (1) human HeLa cell, (2) human HEK 293 cell, (3) human A-431 cell, (4) human HepG2 cell, (6) mouse NIH-3T3 cell, (7) rat cell line NBT-II.
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
  • Immunohistochemical staining (Formalin-fixed paraffin-embedded sections) of human placenta shows strong cytoplasmic immunoreactivity in trophoblasts.
  • RNAi Knockdown
  • RNAi Knockdown
  • Western Blot analysis of U-251cell transfected with (1) control siRNA, (2) target specific siRNA probe #1, (3) target specific siRNA probe #2 using PPIB monoclonal antibody, clone CL3901. Downregulation of antibody signal confirms target specificity. Remaining % intensity, relative control lane, is indicated. GAPDH monoclonal antibody was used as loading control.
  • Application Image
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
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  • Gene Information
  • Entrez GeneID:
  • 5479
  • Gene Name:
  • PPIB
  • Gene Alias:
  • CYP-S1,CYPB,MGC14109,MGC2224,SCYLP
  • Gene Description:
  • peptidylprolyl isomerase B (cyclophilin B)
  • Gene Summary:
  • The protein encoded by this gene is a cyclosporine-binding protein and is mainly located within the endoplasmic reticulum. It is associated with the secretory pathway and released in biological fluids. This protein can bind to cells derived from T- and B-lymphocytes, and may regulate cyclosporine A-mediated immunosuppression. [provided by RefSeq
  • Other Designations:
  • PPIase,S-cyclophilin,cyclophilin B,cyclophilin-like protein,peptidyl-prolyl cis-trans isomerase B,peptidylprolyl isomerase B,rotamase
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