PPIB monoclonal antibody, clone CL3901
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Specification
Product Description
Mouse monoclonal antibody raised against synthetic peptide of human PPIB.
Immunogen
A synthetic peptide corresponding to human PPIB.
Sequence
TKTDSRDKPLKDVIIADCGKIEVEKPFAIAKE
Host
Mouse
Reactivity
Human, Mouse, Rat
Form
Liquid
Purification
Protein A purification
Isotype
IgG2a
Recommend Usage
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) (1:5000-1:10000)
Western Blot (1:500-1:1000)
The optimal working dilution should be determined by the end user.Storage Buffer
In PBS, pH 7.2 (40% glycerol, 0.02% sodium azide).
Storage Instruction
Store at 4°C. For long term storage store at -20°C.
Aliquot to avoid repeated freezing and thawing.Note
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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Applications
Western Blot
Western Blot (Cell lysate) analysis of (1) human HeLa cell, (2) human HEK 293 cell, (3) human A-431 cell, (4) human HepG2 cell, (6) mouse NIH-3T3 cell, (7) rat cell line NBT-II.Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Immunohistochemical staining (Formalin-fixed paraffin-embedded sections) of human placenta shows strong cytoplasmic immunoreactivity in trophoblasts.RNAi Knockdown
Western Blot analysis of U-251cell transfected with (1) control siRNA, (2) target specific siRNA probe #1, (3) target specific siRNA probe #2 using PPIB monoclonal antibody, clone CL3901. Downregulation of antibody signal confirms target specificity. Remaining % intensity, relative control lane, is indicated. GAPDH monoclonal antibody was used as loading control. -
Gene Info — PPIB
Entrez GeneID
5479Protein Accession#
P23284Gene Name
PPIB
Gene Alias
CYP-S1, CYPB, MGC14109, MGC2224, SCYLP
Gene Description
peptidylprolyl isomerase B (cyclophilin B)
Omim ID
123841Gene Ontology
HyperlinkGene Summary
The protein encoded by this gene is a cyclosporine-binding protein and is mainly located within the endoplasmic reticulum. It is associated with the secretory pathway and released in biological fluids. This protein can bind to cells derived from T- and B-lymphocytes, and may regulate cyclosporine A-mediated immunosuppression. [provided by RefSeq
Other Designations
PPIase|S-cyclophilin|cyclophilin B|cyclophilin-like protein|peptidyl-prolyl cis-trans isomerase B|peptidylprolyl isomerase B|rotamase
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Interactome
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Disease
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