ACTB monoclonal antibody, clone CL3868
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More Files
- More Functions
Western Blot
Western Blot (Cell lysate) analysis of (1) Human HeLa cell, (2) Human HEK 293 cell, (3) Human A-431 cell, (4) Human HepG2 cell. (5) Mouse NIH-3T3 cell, (6) Rat NBT-II cell.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Immunohistochemical staining (Formalin-fixed paraffin-embedded sections) of human prostate shows strong cytoplasmic immunoreactivity in smooth muscle and weak positivity in glandular cells.
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Specification
Product Description
Mouse monoclonal antibody raised against synthetic peptide of human ACTB.
Immunogen
A synthetic peptide corresponding to human ACTB.
Sequence
PKANREKMTQIMFETFNTPAMYVAIQ
Host
Mouse
Reactivity
Human, Mouse, Rat
Form
Liquid
Purification
Protein A purification
Isotype
IgG1
Recommend Usage
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) (1:5000-1:10000)
Western Blot (1:500-1:1000)
The optimal working dilution should be determined by the end user.Storage Buffer
In PBS, pH 7.2 (40% glycerol, 0.02% sodium azide).
Storage Instruction
Store at 4°C. For long term storage store at -20°C.
Aliquot to avoid repeated freezing and thawing.Note
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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Applications
Western Blot
Western Blot (Cell lysate) analysis of (1) Human HeLa cell, (2) Human HEK 293 cell, (3) Human A-431 cell, (4) Human HepG2 cell. (5) Mouse NIH-3T3 cell, (6) Rat NBT-II cell.Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Immunohistochemical staining (Formalin-fixed paraffin-embedded sections) of human prostate shows strong cytoplasmic immunoreactivity in smooth muscle and weak positivity in glandular cells. -
Gene Info — ACTB
Entrez GeneID
60Protein Accession#
P60709Gene Name
ACTB
Gene Alias
PS1TP5BP1
Gene Description
actin, beta
Omim ID
102630Gene Ontology
HyperlinkGene Summary
This gene encodes one of six different actin proteins. Actins are highly conserved proteins that are involved in cell motility, structure, and integrity. This actin is a major constituent of the contractile apparatus and one of the two nonmuscle cytoskeletal actins. [provided by RefSeq
Other Designations
PS1TP5-binding protein 1|actin, cytoplasmic 1|beta actin|beta cytoskeletal actin
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Interactome
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Pathway
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Disease
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