ERCC1 monoclonal antibody, clone CL1284
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Specification
Product Description
Mouse monoclonal antibody raised against partial recombinant human ERCC1.
Immunogen
Recombinant protein corresponding to human ERCC1.
Epitope
This antibody binds to an epitope located within the peptide sequence KARRLFDVLH as determined by overlapping synthetic peptides.
Sequence
LADCTLILAWSPEEAGRYLETYKAYEQKPADLLMEKLEQDFVSRVTECLTTVKSVNKTDSQTLLTTFGSLEQLIAASREDLALCPGLGPQKARRLFDVLHEPFLKVP
Host
Mouse
Reactivity
Human
Form
Liquid
Purification
Protein A purification
Isotype
IgG1
Recommend Usage
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) (1:200-1:500)
Western Blot (1:500-1:1000)
The optimal working dilution should be determined by the end user.Storage Buffer
In PBS, pH 7.2 (40% glycerol, 0.02% sodium azide).
Storage Instruction
Store at 4°C. For long term storage store at -20°C.
Aliquot to avoid repeated freezing and thawing.Note
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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Applications
Western Blot (Cell lysate)
Western Blot analysis of RT-4 cell lysate with ERCC1 monoclonal antibody, clone CL1284 (Cat # MAB15677).Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Immunohistochemical staining (Formalin-fixed paraffin-embedded sections) of human prostate cancer with ERCC1 monoclonal antibody, clone CL1284 (Cat # MAB15677) shows strong nuclear immunoreactivity in tumor cells. -
Gene Info — ERCC1
Entrez GeneID
2067Protein Accession#
P07992Gene Name
ERCC1
Gene Alias
COFS4, UV20
Gene Description
excision repair cross-complementing rodent repair deficiency, complementation group 1 (includes overlapping antisense sequence)
Gene Ontology
HyperlinkGene Summary
O
Other Designations
excision repair cross-complementing 1|excision repair protein
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Interactome
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Pathway
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Disease
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