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KDM5B monoclonal antibody, clone CL1129

  • Catalog # : MAB15669
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  • Specification
  • Product Description:
  • Mouse monoclonal antibody raised against partial recombinant human KDM5B.
  • Immunogen:
  • Recombinant protein corresponding to human KDM5B.
  • Sequence:
  • DGINSLERKLKRRLEREGLSSERWERVKKMRTPKKKKIKLSHPKDMNNFKLERERSYELVRSAETHSLPSDTSYSEQEDSE
  • Host:
  • Mouse
  • Reactivity:
  • Human
  • Form:
  • Liquid
  • Purification:
  • Protein A purification
  • Isotype:
  • IgG1
  • Recommend Usage:
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) (1:200-1:500)
    Western Blot (1:500-1:1000)
    The optimal working dilution should be determined by the end user.
  • Storage Buffer:
  • In PBS, pH 7.2 (40% glycerol, 0.02% sodium azide).
  • Storage Instruction:
  • Store at 4°C. For long term storage store at -20°C.
    Aliquot to avoid repeated freezing and thawing.
  • Note:
  • This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
  • Epitope:
  • This antibody binds to an epitope located within the peptide sequence DGINSLERKLKRRLE as determined by overlapping synthetic peptides.
  • Applications
  • Western Blot (Transfected lysate)
  • Western Blot (Transfected lysate)
  • Western Blot analysis of Lane 1: negative control (vector only transfected HEK293T cell lysate) and Lane 2: over-expression lysate (co-expressed with a C-terminal myc-DDK tag in mammalian HEK293T cells) with KDM5B monoclonal antibody, clone CL1129 (Cat # MAB15669).
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
  • Immunohistochemical staining (Formalin-fixed paraffin-embedded sections) of human breast cancer with KDM5B monoclonal antibody, clone CL1129 (Cat # MAB15669) shows strong nuclear immunoreactivity in tumor cells.
  • Application Image
  • Western Blot (Transfected lysate)
  • Western Blot (Transfected lysate)
  • enlarge
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
  • enlarge
  • Gene Information
  • Gene Name:
  • KDM5B
  • Gene Alias:
  • FLJ10538,FLJ12459,FLJ12491,FLJ16281,FLJ23670,KDM5B,PLU-1,PUT1,RBBP2H1A
  • Gene Description:
  • jumonji, AT rich interactive domain 1B
  • Other Designations:
  • OTTHUMP00000038815,RBP2-like,putative DNA/chromatin binding motif,retinoblastoma-binding protein 2, homolog 1A
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