KDM5B monoclonal antibody, clone CL1129
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More Files
- More Functions
Western Blot (Transfected lysate)
Western Blot analysis of Lane 1: negative control (vector only transfected HEK293T cell lysate) and Lane 2: over-expression lysate (co-expressed with a C-terminal myc-DDK tag in mammalian HEK293T cells) with KDM5B monoclonal antibody, clone CL1129 (Cat # MAB15669).
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Immunohistochemical staining (Formalin-fixed paraffin-embedded sections) of human breast cancer with KDM5B monoclonal antibody, clone CL1129 (Cat # MAB15669) shows strong nuclear immunoreactivity in tumor cells.
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Specification
Product Description
Mouse monoclonal antibody raised against partial recombinant human KDM5B.
Immunogen
Recombinant protein corresponding to human KDM5B.
Epitope
This antibody binds to an epitope located within the peptide sequence DGINSLERKLKRRLE as determined by overlapping synthetic peptides.
Sequence
DGINSLERKLKRRLEREGLSSERWERVKKMRTPKKKKIKLSHPKDMNNFKLERERSYELVRSAETHSLPSDTSYSEQEDSE
Host
Mouse
Reactivity
Human
Form
Liquid
Purification
Protein A purification
Isotype
IgG1
Recommend Usage
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) (1:200-1:500)
Western Blot (1:500-1:1000)
The optimal working dilution should be determined by the end user.Storage Buffer
In PBS, pH 7.2 (40% glycerol, 0.02% sodium azide).
Storage Instruction
Store at 4°C. For long term storage store at -20°C.
Aliquot to avoid repeated freezing and thawing.Note
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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Applications
Western Blot (Transfected lysate)
Western Blot analysis of Lane 1: negative control (vector only transfected HEK293T cell lysate) and Lane 2: over-expression lysate (co-expressed with a C-terminal myc-DDK tag in mammalian HEK293T cells) with KDM5B monoclonal antibody, clone CL1129 (Cat # MAB15669).Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Immunohistochemical staining (Formalin-fixed paraffin-embedded sections) of human breast cancer with KDM5B monoclonal antibody, clone CL1129 (Cat # MAB15669) shows strong nuclear immunoreactivity in tumor cells. -
Gene Info — KDM5B
Entrez GeneID
10765Protein Accession#
Q9UGL1Gene Name
KDM5B
Gene Alias
FLJ10538, FLJ12459, FLJ12491, FLJ16281, FLJ23670, KDM5B, PLU-1, PUT1, RBBP2H1A
Gene Description
jumonji, AT rich interactive domain 1B
Omim ID
605393Gene Ontology
HyperlinkGene Summary
O
Other Designations
OTTHUMP00000038815|RBP2-like|putative DNA/chromatin binding motif|retinoblastoma-binding protein 2, homolog 1A
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Interactome
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Disease
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