ATRX monoclonal antibody, clone CL0537
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More Files
- More Functions
Western Blot (Cell lysate)
Western Blot analysis of A-549 cell lysate with ATRX monoclonal antibody, clone CL0537 (Cat # MAB15638).
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Immunohistochemical staining (Formalin-fixed paraffin-embedded sections) of human glioma with ATRX monoclonal antibody, clone CL0537 (Cat # MAB15638) shows strong nuclear positivity in tumor cells.
Immunofluorescence
Immunofluorescent staining of HeLa cells with ATRX monoclonal antibody, clone CL0537 (Cat # MAB15638) (Green) shows clear nuclear (without nucleoli) staining. Microtubule and nuclear probes are visualized in red and blue respectively (where available).
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Specification
Product Description
Mouse monoclonal antibody raised against partial recombinant human ATRX.
Immunogen
Recombinant protein corresponding to human ATRX.
Sequence
AAWAEYEAEKKGLTMRFNIPTGTNLPPVSFNSQTPYIPFNLGALSAMSNQQLEDLINQGREKVVEATNSVTAVRIQPLEDIISAVWKENMNLSEAQVQALALSRQASQELDVKRREAIYNDVLTKQQMLISCVQRILMNRR
Host
Mouse
Reactivity
Human
Form
Liquid
Purification
Protein A purification
Isotype
IgG1
Recommend Usage
Immunofluorescence (1-4 ug/mL)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) (1:200-1:500)
Western Blot (1:500-1:1000)
The optimal working dilution should be determined by the end user.Storage Buffer
In PBS, pH 7.2 (40% glycerol, 0.02% sodium azide).
Storage Instruction
Store at 4°C. For long term storage store at -20°C.
Aliquot to avoid repeated freezing and thawing.Note
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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Applications
Western Blot (Cell lysate)
Western Blot analysis of A-549 cell lysate with ATRX monoclonal antibody, clone CL0537 (Cat # MAB15638).Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Immunohistochemical staining (Formalin-fixed paraffin-embedded sections) of human glioma with ATRX monoclonal antibody, clone CL0537 (Cat # MAB15638) shows strong nuclear positivity in tumor cells.Immunofluorescence
Immunofluorescent staining of HeLa cells with ATRX monoclonal antibody, clone CL0537 (Cat # MAB15638) (Green) shows clear nuclear (without nucleoli) staining. Microtubule and nuclear probes are visualized in red and blue respectively (where available). -
Gene Info — ATRX
Entrez GeneID
546Protein Accession#
P46100Gene Name
ATRX
Gene Alias
ATR2, MGC2094, MRXHF1, RAD54, RAD54L, SFM1, SHS, XH2, XNP, ZNF-HX
Gene Description
alpha thalassemia/mental retardation syndrome X-linked (RAD54 homolog, S. cerevisiae)
Gene Ontology
HyperlinkGene Summary
The protein encoded by this gene contains an ATPase/helicase domain, and thus it belongs to the SWI/SNF family of chromatin remodeling proteins. The mutations of this gene are associated with an X-linked mental retardation (XLMR) syndrome most often accompanied by alpha-thalassemia (ATRX) syndrome. These mutations have been shown to cause diverse changes in the pattern of DNA methylation, which may provide a link between chromatin remodeling, DNA methylation, and gene expression in developmental processes. This protein is found to undergo cell cycle-dependent phosphorylation, which regulates its nuclear matrix and chromatin association, and suggests its involvement in the gene regulation at interphase and chromosomal segregation in mitosis. Multiple alternatively spliced transcript variants encoding distinct isoforms have been reported. [provided by RefSeq
Other Designations
DNA dependent ATPase and helicase|OTTHUMP00000024265|OTTHUMP00000062079|X-linked nuclear protein|Zinc finger helicase|helicase 2, X-linked|transcriptional regulator ATRX
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Interactome
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Disease
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