P4HA2 monoclonal antibody, clone CL0351
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More Files
- More Functions
Western Blot (Cell lysate)
Western Blot analysis of U-251 MG cell lysate with P4HA2 monoclonal antibody, clone CL0351 (Cat # MAB15614).
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Immunohistochemical staining (Formalin-fixed paraffin-embedded sections) of human endometrium with P4HA2 monoclonal antibody, clone CL0351 (Cat # MAB15614) shows strong cytoplasmic immunoreactivity in glandular epithelium, as well as in underlying connective tissue.
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Specification
Product Description
Mouse monoclonal antibody raised against partial recombinant human P4HA2.
Immunogen
Recombinant protein corresponding to human P4HA2.
Epitope
This antibody binds to an epitope located within the peptide sequence NTDWPALEDLVLQDS as determined by overlapping synthetic peptides.
Sequence
LKEYILVEEAKLSKIKSWANKMEALTSKSAADAEGYLAHPVNAYKLVKRLNTDWPALEDLVLQDSAAGFIANLSVQRQFFPTDEDEIGAAKALMRLQDTYRLDPGTISRGELPGTKYQAMLSVDDCFGM
Host
Mouse
Reactivity
Human
Form
Liquid
Purification
Protein A purification
Isotype
IgG1
Recommend Usage
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) (1:200-1:500)
Western Blot (1:500-1:1000)
The optimal working dilution should be determined by the end user.Storage Buffer
In PBS, pH 7.2 (40% glycerol, 0.02% sodium azide).
Storage Instruction
Store at 4°C. For long term storage store at -20°C.
Aliquot to avoid repeated freezing and thawing.Note
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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Applications
Western Blot (Cell lysate)
Western Blot analysis of U-251 MG cell lysate with P4HA2 monoclonal antibody, clone CL0351 (Cat # MAB15614).Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Immunohistochemical staining (Formalin-fixed paraffin-embedded sections) of human endometrium with P4HA2 monoclonal antibody, clone CL0351 (Cat # MAB15614) shows strong cytoplasmic immunoreactivity in glandular epithelium, as well as in underlying connective tissue. -
Gene Info — P4HA2
Entrez GeneID
8974Protein Accession#
O15460Gene Name
P4HA2
Gene Alias
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Gene Description
prolyl 4-hydroxylase, alpha polypeptide II
Omim ID
600608Gene Ontology
HyperlinkGene Summary
This gene encodes a component of prolyl 4-hydroxylase, a key enzyme in collagen synthesis composed of two identical alpha subunits and two beta subunits. The encoded protein is one of several different types of alpha subunits and provides the major part of the catalytic site of the active enzyme. In collagen and related proteins, prolyl 4-hydroxylase catalyzes the formation of 4-hydroxyproline that is essential to the proper three-dimensional folding of newly synthesized procollagen chains. Alternatively spliced transcript variants encoding different isoforms have been described. [provided by RefSeq
Other Designations
4-PH alpha 2|C-P4Halpha(II)|OTTHUMP00000065966|OTTHUMP00000065967|OTTHUMP00000065968|OTTHUMP00000065969|collagen prolyl 4-hydroxylase alpha(II)|procollagen-proline, 2-oxoglutarate 4-dioxygenase (proline 4-hydroxylase), alpha polypeptide II|prolyl 4-hydrox
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Interactome
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Pathway
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Disease
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