ANLN monoclonal antibody, clone CL0303
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More Files
- More Functions
Western Blot (Cell lysate)
Western Blot analysis of U-251 MG cell lysate with ANLN monoclonal antibody, clone CL0303 (Cat # MAB15596).
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Immunohistochemical staining (Formalin-fixed paraffin-embedded sections) of human lung cancer with ANLN monoclonal antibody, clone CL0303 (Cat # MAB15596) shows strong nuclear positivity in a subset of tumor cells.
Immunofluorescence
Immunofluorescent staining of HeLa cells with ANLN monoclonal antibody, clone CL0303 (Cat # MAB15596) (Green) shows cell cycle dependent nuclear (without nucleoli) staining. Microtubule and nuclear probes are visualized in red and blue respectively (where available).
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Specification
Product Description
Mouse monoclonal antibody raised against partial recombinant human ANLN.
Immunogen
Recombinant protein corresponding to human ANLN.
Epitope
This antibody binds to an epitope located within the peptide sequence LRGRFDKGNIWSAEK as determined by overlapping synthetic peptides.
Sequence
IVKSTLSQTVPSKGELSREICLQSQSKDKSTTPGGTGIKPFLERFGERCQEHSKESPARSTPHRTPIITPNTKAIQERLFKQDTSSSTTHLAQQLKQERQKELACLRGRFDKGNIWSAEKGGNSKSKQLETKQETH
Host
Mouse
Reactivity
Human
Form
Liquid
Purification
Protein A purification
Isotype
IgG1
Recommend Usage
Immunofluorescence (1-4 ug/mL)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) (1:50-1:200)
Western Blot (1:500-1:1000)
The optimal working dilution should be determined by the end user.Storage Buffer
In PBS, pH 7.2 (40% glycerol, 0.02% sodium azide).
Storage Instruction
Store at 4°C. For long term storage store at -20°C.
Aliquot to avoid repeated freezing and thawing.Note
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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Applications
Western Blot (Cell lysate)
Western Blot analysis of U-251 MG cell lysate with ANLN monoclonal antibody, clone CL0303 (Cat # MAB15596).Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Immunohistochemical staining (Formalin-fixed paraffin-embedded sections) of human lung cancer with ANLN monoclonal antibody, clone CL0303 (Cat # MAB15596) shows strong nuclear positivity in a subset of tumor cells.Immunofluorescence
Immunofluorescent staining of HeLa cells with ANLN monoclonal antibody, clone CL0303 (Cat # MAB15596) (Green) shows cell cycle dependent nuclear (without nucleoli) staining. Microtubule and nuclear probes are visualized in red and blue respectively (where available). -
Gene Info — ANLN
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Interactome
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