SATB2 monoclonal antibody, clone CL0276
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Specification
Product Description
Mouse monoclonal antibody raised against partial recombinant human SATB2.
Immunogen
Recombinant protein corresponding to human SATB2.
Epitope
This antibody binds to an epitope located within the peptide sequence LVNLRAMQNFLNLPE as determined by overlapping synthetic peptides.
Sequence
VSQAVFARVAFNRTQGLLSEILRKEEDPRTASQSLLVNLRAMQNFLNLPEVERDRIYQDERERSMNPNVSMVSSASSSPSSSRTPQAKTSTPTTDLPIKVDGANINITAAIYDEIQQEMKRAK
Host
Mouse
Reactivity
Human
Form
Liquid
Purification
Protein A purification
Isotype
IgG1
Recommend Usage
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) (1:200-1:500)
Western Blot (1:500-1:1000)
The optimal working dilution should be determined by the end user.Storage Buffer
In PBS, pH 7.2 (40% glycerol, 0.02% sodium azide).
Storage Instruction
Store at 4°C. For long term storage store at -20°C.
Aliquot to avoid repeated freezing and thawing.Note
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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Applications
Western Blot (Cell lysate)
Western Blot analysis of HEL cell lysate with SATB2 monoclonal antibody, clone CL0276 (Cat # MAB15588).Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Immunohistochemical staining (Formalin-fixed paraffin-embedded sections) of human rectum with SATB2 monoclonal antibody, clone CL0276 (Cat # MAB15588) shows strong nuclear positivity in glandular cells. -
Gene Info — SATB2
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Interactome
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Disease
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Publication Reference
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Evaluating real-time immunohistochemistry on multiple tissue samples, multiple targets and multiple antibody labeling methods.
Dubois L, Andersson K, Asplund A, Björkelund H.
BMC Research Notes 2013 Dec; 6:542.
Application:IHC-P, Human, Mouse, Colon, Heart muscle, Liver, Tonsil.
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Scalable in situ hybridization on tissue arrays for validation of novel cancer and tissue-specific biomarkers.
Kiflemariam S, Andersson S, Asplund A, Pontén F, Sjöblom T.
PLoS One 2012 Mar; 7(3):e32927.
Application:IHC-P, Human, Human tissue microarray.
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A cohort study of the prognostic and treatment predictive value of SATB2 expression in colorectal cancer.
Eberhard J, Gaber A, Wangefjord S, Nodin B, Uhlén M, Ericson Lindquist K, Jirström K.
British Journal of Cancer 2012 Feb; 106(5):931.
Application:IHC-P, Human, Human colorectal cancer, TMA sections.
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Epitope mapping of antibodies using bacterial surface display.
Rockberg J, Löfblom J, Hjelm B, Uhlén M, Ståhl S.
Nature Methods 2008 Dec; 5(12):1039.
Application:Flow Cyt, Bacteria, Staphylococcal cells.
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Evaluating real-time immunohistochemistry on multiple tissue samples, multiple targets and multiple antibody labeling methods.
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