HMGCR monoclonal antibody, clone CL0259
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Specification
Product Description
Mouse monoclonal antibody raised against partial recombinant human HMGCR.
Immunogen
Recombinant protein corresponding to human HMGCR.
Epitope
This antibody binds to an epitope located within the peptide sequence TLMETHERGVSIRRQ as determined by overlapping synthetic peptides.
Sequence
HIPAYKLETLMETHERGVSIRRQLLSKKLSEPSSLQYLPYRDYNYSLVMGACCENVIGYMPIPVGVAGPLCLDEKEFQVPMATTEGCLVA
Host
Mouse
Reactivity
Human
Form
Liquid
Purification
Protein A purification
Isotype
IgG2a
Recommend Usage
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) (1:50-1:200)
Western Blot (1:500-1:1000)
The optimal working dilution should be determined by the end user.Storage Buffer
In PBS, pH 7.2 (40% glycerol, 0.02% sodium azide).
Storage Instruction
Store at 4°C. For long term storage store at -20°C.
Aliquot to avoid repeated freezing and thawing.Note
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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Applications
Western Blot (Cell lysate)
Western Blot analysis of Lane 1: MCF-7 and Lane 2: HepG2 cell lysates with HMGCR monoclonal antibody, clone CL0259 (Cat # MAB15579).Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Immunohistochemical staining (Formalin-fixed paraffin-embedded sections) of human liver with HMGCR monoclonal antibody, clone CL0259 (Cat # MAB15579) shows strong cytoplasmic immunoreactivity. -
Gene Info — HMGCR
Entrez GeneID
3156Protein Accession#
P04035Gene Name
HMGCR
Gene Alias
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Gene Description
3-hydroxy-3-methylglutaryl-Coenzyme A reductase
Omim ID
142910Gene Ontology
HyperlinkGene Summary
HMG-CoA reductase is the rate-limiting enzyme for cholesterol synthesis and is regulated via a negative feedback mechanism mediated by sterols and non-sterol metabolites derived from mevalonate, the product of the reaction catalyzed by reductase. Normally in mammalian cells this enzyme is suppressed by cholesterol derived from the internalization and degradation of low density lipoprotein (LDL) via the LDL receptor. Competitive inhibitors of the reductase induce the expression of LDL receptors in the liver, which in turn increases the catabolism of plasma LDL and lowers the plasma concentration of cholesterol, an important determinant of atherosclerosis. Alternatively spliced transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq
Other Designations
3-hydroxy-3-methylglutaryl CoA reductase (NADPH)|hydroxymethylglutaryl-CoA reductase
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Interactome
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