RNASE7 monoclonal antibody, clone CL0223
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More Files
- More Functions
Western Blot (Transfected lysate)
Western Blot analysis of Lane 1: negative control (vector only transfected HEK293T cell lysate) and Lane 2: over-expression lysate (co-expressed with a C-terminal myc-DDK tag in mammalian HEK293T cells) with RNASE7 monoclonal antibody, clone CL0223 (Cat # MAB15567).
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Immunohistochemical staining (Formalin-fixed paraffin-embedded sections) of human skin with RNASE7 monoclonal antibody, clone CL0223 (Cat # MAB15567) shows strong immunoreactivity in the outer layer of keratinized squamous epithelium.
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Specification
Product Description
Mouse monoclonal antibody raised against partial recombinant human RNASE7.
Immunogen
Recombinant protein corresponding to human RNASE7.
Epitope
This antibody binds to an epitope located within the peptide sequence LTMCKLTSGKYPNCR as determined by overlapping synthetic peptides.
Sequence
KGMTSSQWFKIQHMQPSPQACNSAMKNINKHTKRCKDLNTFLHEPFSSVAATCQTPKIACKNGDKNCHQSHGPVSLTMCKLTSGKYPNCRYKEKRQNKSYVVACKPPQKKDSQQFHLVPVHLDRV
Host
Mouse
Reactivity
Human
Form
Liquid
Purification
Protein A purification
Isotype
IgG1
Recommend Usage
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) (1:50-1:200)
Western Blot (1:500-1:1000)
The optimal working dilution should be determined by the end user.Storage Buffer
In PBS, pH 7.2 (40% glycerol, 0.02% sodium azide).
Storage Instruction
Store at 4°C. For long term storage store at -20°C.
Aliquot to avoid repeated freezing and thawing.Note
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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Applications
Western Blot (Transfected lysate)
Western Blot analysis of Lane 1: negative control (vector only transfected HEK293T cell lysate) and Lane 2: over-expression lysate (co-expressed with a C-terminal myc-DDK tag in mammalian HEK293T cells) with RNASE7 monoclonal antibody, clone CL0223 (Cat # MAB15567).Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Immunohistochemical staining (Formalin-fixed paraffin-embedded sections) of human skin with RNASE7 monoclonal antibody, clone CL0223 (Cat # MAB15567) shows strong immunoreactivity in the outer layer of keratinized squamous epithelium. -
Gene Info — RNASE7
Entrez GeneID
84659Protein Accession#
Q9H1E1Gene Name
RNASE7
Gene Alias
MGC133220
Gene Description
ribonuclease, RNase A family, 7
Gene Ontology
HyperlinkGene Summary
RNASE7 is a member of the RNase A superfamily that has broad-spectrum antimicrobial activity and ribonuclease activity (Harder and Schroder, 2002 [PubMed 12244054]; Zhang et al., 2003 [PubMed 12527768]).[supplied by OMIM
Other Designations
ribonuclease 7
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