EMD monoclonal antibody, clone CL0203

Catalog # MAB15560

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Size:100 uL
Price: USD $ 428.00
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Contact Info
  • +1-909-264-1399
    +1-909-992-0619
    Toll Free : +1-877-853-6098
  • +1-909-992-3401
Images
Western Blot (Cell lysate)
Application

Western Blot (Cell lysate)

Western Blot analysis of RT-4 cell lysate with EMD monoclonal antibody, clone CL0203 (Cat # MAB15560).

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Application

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)

Immunohistochemical staining (Formalin-fixed paraffin-embedded sections) of human skin with EMD monoclonal antibody, clone CL0203 (Cat # MAB15560) shows strong nuclear membrane positivity in the epidermis cells.

Immunofluorescence
Application

Immunofluorescence

Immunofluorescent staining of HeLa cells with EMD monoclonal antibody, clone CL0203 (Cat # MAB15560) (Green) shows a distinct nuclear membranous pattern with a weaker additional ER. Microtubule and nuclear probes are visualized in red and blue respectively (where available).

  • Specification

    Product Description

    Mouse monoclonal antibody raised against partial recombinant human EMD.

    Immunogen

    Recombinant protein corresponding to human EMD.

    Epitope

    This antibody binds to an epitope located within the peptide sequence QSKGYNDDYY as determined by overlapping synthetic peptides.

    Sequence

    ASSYSFSDLNSTRGDADMYDLPKKEDALLYQSKGYNDDYYEESYFTTRTYGEPESAGPSRAVRQSVTSFPDADAFHHQVHDDDLLSSSEEECKDRERPMYGRDSACQSITHY

    Host

    Mouse

    Reactivity

    Human

    Form

    Liquid

    Purification

    Protein A purification

    Isotype

    IgG1

    Recommend Usage

    Immunofluorescence (1-4 ug/mL)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) (1:5000-1:10000)
    Western Blot (1:100-1:250)
    The optimal working dilution should be determined by the end user.

    Storage Buffer

    In PBS, pH 7.2 (40% glycerol, 0.02% sodium azide).

    Storage Instruction

    Store at 4°C. For long term storage store at -20°C.
    Aliquot to avoid repeated freezing and thawing.

    Note

    This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

  • Applications

    Western Blot (Cell lysate)

    Western Blot analysis of RT-4 cell lysate with EMD monoclonal antibody, clone CL0203 (Cat # MAB15560).

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)

    Immunohistochemical staining (Formalin-fixed paraffin-embedded sections) of human skin with EMD monoclonal antibody, clone CL0203 (Cat # MAB15560) shows strong nuclear membrane positivity in the epidermis cells.

    Immunofluorescence

    Immunofluorescent staining of HeLa cells with EMD monoclonal antibody, clone CL0203 (Cat # MAB15560) (Green) shows a distinct nuclear membranous pattern with a weaker additional ER. Microtubule and nuclear probes are visualized in red and blue respectively (where available).
  • Gene Info — EMD

    Entrez GeneID

    2010

    Protein Accession#

    P50402

    Gene Name

    EMD

    Gene Alias

    EDMD, LEMD5, STA

    Gene Description

    emerin

    Omim ID

    300384 310300

    Gene Ontology

    Hyperlink

    Gene Summary

    Emerin is a serine-rich nuclear membrane protein and a member of the nuclear lamina-associated protein family. It mediates membrane anchorage to the cytoskeleton. Dreifuss-Emery muscular dystrophy is an X-linked inherited degenerative myopathy resulting from mutation in the emerin gene. [provided by RefSeq

    Other Designations

    LEM domain containing 5|OTTHUMP00000031938|OTTHUMP00000061687

  • Interactome
  • Pathway
  • Disease
Contact Info
  • +1-909-264-1399
    +1-909-992-0619
    Toll Free : +1-877-853-6098
  • +1-909-992-3401
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