EMD monoclonal antibody, clone CL0201
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Specification
Product Description
Mouse monoclonal antibody raised against partial recombinant human EMD.
Immunogen
Recombinant protein corresponding to human EMD.
Epitope
This antibody binds to an epitope located within the peptide sequence DALLYQSKGYNDDYY as determined by overlapping synthetic peptides.
Sequence
ASSYSFSDLNSTRGDADMYDLPKKEDALLYQSKGYNDDYYEESYFTTRTYGEPESAGPSRAVRQSVTSFPDADAFHHQVHDDDLLSSSEEECKDRERPMYGRDSACQSITHY
Host
Mouse
Reactivity
Human
Form
Liquid
Purification
Protein A purification
Isotype
IgG2a
Recommend Usage
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) (1:2500-1:5000)
Western Blot (1:500-1:1000)
The optimal working dilution should be determined by the end user.Storage Buffer
In PBS, pH 7.2 (40% glycerol, 0.02% sodium azide).
Storage Instruction
Store at 4°C. For long term storage store at -20°C.
Aliquot to avoid repeated freezing and thawing.Note
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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Applications
Western Blot (Cell lysate)
Western Blot analysis of RT-4 cell lysate with EMD monoclonal antibody, clone CL0201 (Cat # MAB15559).Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Immunohistochemical staining (Formalin-fixed paraffin-embedded sections) of human fallopian tube with EMD monoclonal antibody, clone CL0201 (Cat # MAB15559) shows strong positivity in the nuclear membrane of glandular cells. -
Gene Info — EMD
Entrez GeneID
2010Protein Accession#
P50402Gene Name
EMD
Gene Alias
EDMD, LEMD5, STA
Gene Description
emerin
Gene Ontology
HyperlinkGene Summary
Emerin is a serine-rich nuclear membrane protein and a member of the nuclear lamina-associated protein family. It mediates membrane anchorage to the cytoskeleton. Dreifuss-Emery muscular dystrophy is an X-linked inherited degenerative myopathy resulting from mutation in the emerin gene. [provided by RefSeq
Other Designations
LEM domain containing 5|OTTHUMP00000031938|OTTHUMP00000061687
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Interactome
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