SIX1 monoclonal antibody, clone CL0185
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More Files
- More Functions
Western Blot (Cell lysate)
Western Blot analysis of Lane 1: RH-30 and Lane 2: CACO-2 cell lysates with SIX1 monoclonal antibody, clone CL0185 (Cat # MAB15554).
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Immunohistochemical staining (Formalin-fixed paraffin-embedded sections) of human striated muscle with SIX1 monoclonal antibody, clone CL0185 (Cat # MAB15554) shows strong nuclear immunoreactivity in the myocytes.
Immunofluorescence
Immunofluorescent staining of RH30 cells with SIX1 monoclonal antibody, clone CL0185 (Cat # MAB15554) (Green) shows spotty nuclear. Microtubule probes are visualized in red (where available).
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Specification
Product Description
Mouse monoclonal antibody raised against partial recombinant human SIX1.
Immunogen
Recombinant protein corresponding to human SIX1.
Sequence
CFKEKSRGVLREWYAHNPYPSPREKRELAEATGLTTTQVSNWFKNRRQRDRAAEAKERENTENNNSSSNKQNQLSPLEGGKPLMSSSEEEFSPPQSPDQNSVLLLQGNMGHARSSNYSLPGLTASQPSHGLQTHQHQLQDS
Host
Mouse
Reactivity
Human
Form
Liquid
Purification
Protein A purification
Isotype
IgG1
Recommend Usage
Immunofluorescence (1-4 ug/mL)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) (1:200-1:500)
Western Blot (1:500-1:1000)
The optimal working dilution should be determined by the end user.Storage Buffer
In PBS, pH 7.2 (40% glycerol, 0.02% sodium azide).
Storage Instruction
Store at 4°C. For long term storage store at -20°C.
Aliquot to avoid repeated freezing and thawing.Note
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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Applications
Western Blot (Cell lysate)
Western Blot analysis of Lane 1: RH-30 and Lane 2: CACO-2 cell lysates with SIX1 monoclonal antibody, clone CL0185 (Cat # MAB15554).Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Immunohistochemical staining (Formalin-fixed paraffin-embedded sections) of human striated muscle with SIX1 monoclonal antibody, clone CL0185 (Cat # MAB15554) shows strong nuclear immunoreactivity in the myocytes.Immunofluorescence
Immunofluorescent staining of RH30 cells with SIX1 monoclonal antibody, clone CL0185 (Cat # MAB15554) (Green) shows spotty nuclear. Microtubule probes are visualized in red (where available). -
Gene Info — SIX1
Entrez GeneID
6495Protein Accession#
Q15475Gene Name
SIX1
Gene Alias
BOS3, DFNA23, TIP39
Gene Description
SIX homeobox 1
Gene Ontology
HyperlinkGene Summary
The protein encoded by this gene is a homeobox protein that is similar to the Drosophila 'sine oculis' gene product. This gene is found in a cluster of related genes on chromosome 14 and is thought to be involved in limb development. Defects in this gene are a cause of autosomal dominant deafness type 23 (DFNA23) and branchiootic syndrome type 3 (BOS3). [provided by RefSeq
Other Designations
sine oculis homeobox homolog 1
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